Observation of the three-dimensional structure of actin bundles formed with polycations

Kazuhiro Shikinaka, Hyuckjoon Kwon, Akira Kakugo, Hidemitsu Furukawa, Yoshihito Osada, Jian Ping Gong, Yoshitaka Aoyama, Hideo Nishioka, Hiroshi Jinnai, Takaharu Okajima

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Three-dimensional structures of actin bundles formed with polycations were observed by using transmission electron microtomography and atomic force microscopy. We found, for the first time, that the cross-sectional morphology of actin bundles depends on the polycation species and ionic strength, while it is insensitive to the degree of polymerization and concentration of polycation. Actin bundles formed with poly-N-[3-(dimethylamino)propyl] acrylamide methyl chloride quaternary show a ribbon-like cross-sectional morphology in low salt concentrations that changes to cylindrical cross-sectional morphology with hexagonal packing of the actin filaments in high salt concentrations. Contrastingly, actin bundles formed with POly-L-lysine show triangular cross-sectional morphology with hexagonal packing of the actin filaments. These variations in cross-sectional morphology are discussed in terms of anisotropy in the electrostatic energy barrier.

Original languageEnglish
Pages (from-to)537-542
Number of pages6
JournalBiomacromolecules
Volume9
Issue number2
DOIs
Publication statusPublished - 2008 Feb 1
Externally publishedYes

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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