TY - CHAP
T1 - Observation of phototropic responses in the liverwort marchantia polymorpha
AU - Komatsu, Aino
AU - Nishihama, Ryuichi
AU - Kohchi, Takayuki
N1 - Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2019.
PY - 2019
Y1 - 2019
N2 - The liverwort species, Marchantia polymorpha, shows environment-dependent morphological plasticity throughout its life cycle. Thalli, representing the predominant body form throughout most of this bryophyte’s life cycle, grow with repeated dichotomous branching at the apex and develop horizontally under sufficient light intensity. Spores, after germination, produce a mass of cells, called sporelings, which then grow into thalli. Both thalli and sporelings, if grown under weak light conditions, form narrow shapes, and their apices grow toward the light source. These phototropic responses are specific to blue light and dependent on the blue-light receptor phototropin. This chapter provides several basic procedures, along with some tips, for designing and performing experiments with M. polymorpha to observe their phototropic responses, as well as methods for observing the localization of the phototropin “Mpphot” with a fluorescent protein tag.
AB - The liverwort species, Marchantia polymorpha, shows environment-dependent morphological plasticity throughout its life cycle. Thalli, representing the predominant body form throughout most of this bryophyte’s life cycle, grow with repeated dichotomous branching at the apex and develop horizontally under sufficient light intensity. Spores, after germination, produce a mass of cells, called sporelings, which then grow into thalli. Both thalli and sporelings, if grown under weak light conditions, form narrow shapes, and their apices grow toward the light source. These phototropic responses are specific to blue light and dependent on the blue-light receptor phototropin. This chapter provides several basic procedures, along with some tips, for designing and performing experiments with M. polymorpha to observe their phototropic responses, as well as methods for observing the localization of the phototropin “Mpphot” with a fluorescent protein tag.
KW - Blue light
KW - Gemmaling
KW - Liverwort
KW - Marchantia polymorpha
KW - Phototropic responses
KW - Phototropin
KW - Sporeling
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U2 - 10.1007/978-1-4939-9015-3_6
DO - 10.1007/978-1-4939-9015-3_6
M3 - Chapter
C2 - 30694467
AN - SCOPUS:85060650575
T3 - Methods in Molecular Biology
SP - 53
EP - 61
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -