TY - JOUR
T1 - Observation of photoactive yellow protein anchored to a modified Au(1 1 1) surface by scanning tunneling microscopy
AU - Rzeźnicka, Izabela I.
AU - Wurpel, George W.H.
AU - Bonn, Mischa
AU - van der Horst, Michael A.
AU - Hellingwerf, Klaas J.
AU - Matsunaga, Soichiro
AU - Yamada, Taro
AU - Kawai, Maki
PY - 2009/4/6
Y1 - 2009/4/6
N2 - The adsorption of photoactive yellow protein (PYP) on a Au(1 1 1) surface and its fluorescence activity have been studied by electrochemical scanning tunneling microscopy (EC-STM) and fluorescence photometry. A stable, densely packed protein layer was observed after protein immobilization onto a Au(1 1 1) surface modified with a mixture of 3-mercaptopropanoic acid (3-MPA) and 11-mercaptoundecanoic acid (11-MUA) and subsequent formation of the amide bond with the use of N-hydroxysuccinimide and carbodiimide. Fluorescence photometry data indicate that covalent binding of PYP to the functionalized Au(1 1 1) surface does not interfere with the fluorescence properties of the native protein.
AB - The adsorption of photoactive yellow protein (PYP) on a Au(1 1 1) surface and its fluorescence activity have been studied by electrochemical scanning tunneling microscopy (EC-STM) and fluorescence photometry. A stable, densely packed protein layer was observed after protein immobilization onto a Au(1 1 1) surface modified with a mixture of 3-mercaptopropanoic acid (3-MPA) and 11-mercaptoundecanoic acid (11-MUA) and subsequent formation of the amide bond with the use of N-hydroxysuccinimide and carbodiimide. Fluorescence photometry data indicate that covalent binding of PYP to the functionalized Au(1 1 1) surface does not interfere with the fluorescence properties of the native protein.
UR - http://www.scopus.com/inward/record.url?scp=62949246806&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=62949246806&partnerID=8YFLogxK
U2 - 10.1016/j.cplett.2009.02.067
DO - 10.1016/j.cplett.2009.02.067
M3 - Article
AN - SCOPUS:62949246806
VL - 472
SP - 113
EP - 117
JO - Chemical Physics Letters
JF - Chemical Physics Letters
SN - 0009-2614
IS - 1-3
ER -