Observation of photoactive yellow protein anchored to a modified Au(1 1 1) surface by scanning tunneling microscopy

Izabela I. Rzeźnicka; George W.H. Wurpel; Mischa Bonn; Michael A. van der Horst; Klaas J. Hellingwerf; Soichiro Matsunaga; Taro Yamada; Maki Kawai

doi:10.1016/j.cplett.2009.02.067

Observation of photoactive yellow protein anchored to a modified Au(1 1 1) surface by scanning tunneling microscopy

Izabela I. Rzeźnicka, George W.H. Wurpel, Mischa Bonn, Michael A. van der Horst, Klaas J. Hellingwerf, Soichiro Matsunaga, Taro Yamada, Maki Kawai

SCI - Chemistry

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The adsorption of photoactive yellow protein (PYP) on a Au(1 1 1) surface and its fluorescence activity have been studied by electrochemical scanning tunneling microscopy (EC-STM) and fluorescence photometry. A stable, densely packed protein layer was observed after protein immobilization onto a Au(1 1 1) surface modified with a mixture of 3-mercaptopropanoic acid (3-MPA) and 11-mercaptoundecanoic acid (11-MUA) and subsequent formation of the amide bond with the use of N-hydroxysuccinimide and carbodiimide. Fluorescence photometry data indicate that covalent binding of PYP to the functionalized Au(1 1 1) surface does not interfere with the fluorescence properties of the native protein.

Original language	English
Pages (from-to)	113-117
Number of pages	5
Journal	Chemical Physics Letters
Volume	472
Issue number	1-3
DOIs	https://doi.org/10.1016/j.cplett.2009.02.067
Publication status	Published - 2009 Apr 6

ASJC Scopus subject areas

Physics and Astronomy(all)
Physical and Theoretical Chemistry

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Observation of photoactive yellow protein anchored to a modified Au(1 1 1) surface by scanning tunneling microscopy. / Rzeźnicka, Izabela I.; Wurpel, George W.H.; Bonn, Mischa; van der Horst, Michael A.; Hellingwerf, Klaas J.; Matsunaga, Soichiro; Yamada, Taro; Kawai, Maki.

In: Chemical Physics Letters, Vol. 472, No. 1-3, 06.04.2009, p. 113-117.

Research output: Contribution to journal › Article › peer-review

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abstract = "The adsorption of photoactive yellow protein (PYP) on a Au(1 1 1) surface and its fluorescence activity have been studied by electrochemical scanning tunneling microscopy (EC-STM) and fluorescence photometry. A stable, densely packed protein layer was observed after protein immobilization onto a Au(1 1 1) surface modified with a mixture of 3-mercaptopropanoic acid (3-MPA) and 11-mercaptoundecanoic acid (11-MUA) and subsequent formation of the amide bond with the use of N-hydroxysuccinimide and carbodiimide. Fluorescence photometry data indicate that covalent binding of PYP to the functionalized Au(1 1 1) surface does not interfere with the fluorescence properties of the native protein.",

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AU - Rzeźnicka, Izabela I.

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AU - van der Horst, Michael A.

AU - Hellingwerf, Klaas J.

AU - Matsunaga, Soichiro

AU - Yamada, Taro

AU - Kawai, Maki

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