Observation of microorganism colonies using a scanning-laser-beam pH-sensing microscope

Motoi Nakao, Shinji Inoue, Ryu Oishi, Tatsuo Yoshinobu, Hiroshi Iwasaki

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The extracellular pH-distribution of colonies of Saccharomyces cerevisiae (yeast) and Escherichia coli (E. coli) were observed using a newly-developed scanning-laser-beam pH-sensing microscope. Colonies were incubated either on top of agarose plates or between the pH-sensing surface and the agar. In the latter case, colony growth was observed in-situ. The colonies could be observed within a period as short as 8 h for E. coli. The pH-distribution profiles by the colonies were found to be very sharp, in agreement with simulation results.

Original languageEnglish
Pages (from-to)163-166
Number of pages4
JournalJournal of Fermentation and Bioengineering
Volume79
Issue number2
DOIs
Publication statusPublished - 1995

Keywords

  • colony-counting
  • metabolism
  • microorganism
  • pH sensor
  • pH-imaging sensor

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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