TY - JOUR
T1 - Observation of DNA pinning at laser focal point on Au surface and its application to single DNA nanowire and cross-wire formation
AU - Fujii, Sho
AU - Kobayashi, Katsuaki
AU - Kanaizuka, Katsuhiko
AU - Okamoto, Tetsuaki
AU - Toyabe, Shoichi
AU - Muneyuki, Eiro
AU - Haga, Masa Aki
N1 - Funding Information:
This work was supported by the “Nanotechnology Support Project” of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan . MH acknowledges the partial financial support received from the Chuo University Joint Research Grant 2009 and the Ministry of Education, Culture, Sports, Science and Technology for a Grant-in-Aid for Priority Area “Coordination Programming” (no. 21108003 ). KK acknowledges the Ministry of Education, Science, Sports, and Culture for a Grant-in-Aid for Scientific Research (no. 19750121 ).
PY - 2010/11
Y1 - 2010/11
N2 - We report a new technique for fabricating a single DNA nanowire at a desired position in a sequential manner using the micronanobubble generated by laser local heating at the Au/water interface. In our previous report, we found the reversible pull-in/shrinkage of one end immobilized DNA strands near a Nd:YAG laser focal point on an Au surface. In further experiments, the pinning of DNA strands in the stretched state was observed on the Au surface only when the bubble has touched the free end of DNA. This pinning phenomenon was observed even on the alkane thiol modified Au surface as self-assembled monolayers (SAMs) such as hexanethiol, mercaptohexanol, and hexadecanethiol. However, no pinning was observed on the bovine serum albumin (BSA) modified surface. Since optical tweezers can manipulate a DNA modified bead (radius. = 1.87. μm), the bead was firstly fixed on a solid surface by being compressed with the optical tweezers, and the pulling and pinning of DNA on the bead were achieved. As a consequence, the laser local heating on the Au surface enables us to control the number and position of the one end immobilized DNA strands as DNA nanowires.
AB - We report a new technique for fabricating a single DNA nanowire at a desired position in a sequential manner using the micronanobubble generated by laser local heating at the Au/water interface. In our previous report, we found the reversible pull-in/shrinkage of one end immobilized DNA strands near a Nd:YAG laser focal point on an Au surface. In further experiments, the pinning of DNA strands in the stretched state was observed on the Au surface only when the bubble has touched the free end of DNA. This pinning phenomenon was observed even on the alkane thiol modified Au surface as self-assembled monolayers (SAMs) such as hexanethiol, mercaptohexanol, and hexadecanethiol. However, no pinning was observed on the bovine serum albumin (BSA) modified surface. Since optical tweezers can manipulate a DNA modified bead (radius. = 1.87. μm), the bead was firstly fixed on a solid surface by being compressed with the optical tweezers, and the pulling and pinning of DNA on the bead were achieved. As a consequence, the laser local heating on the Au surface enables us to control the number and position of the one end immobilized DNA strands as DNA nanowires.
KW - Au surface
KW - DNA nanowire
KW - Laser local heating
KW - Micronano bubble
KW - Single DNA manipulation
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U2 - 10.1016/j.bioelechem.2010.04.004
DO - 10.1016/j.bioelechem.2010.04.004
M3 - Article
C2 - 20537962
AN - SCOPUS:77957903573
VL - 80
SP - 26
EP - 30
JO - Bioelectrochemistry and Bioenergetics
JF - Bioelectrochemistry and Bioenergetics
SN - 1567-5394
IS - 1
ER -