Nutrient concentration-sensitive microorganism-based biosensor

Carl Frederik Werner; Simone Groebel; Christoph Krumbe; Torsten Wagner; Thorsten Selmer; Tatsuo Yoshinobu; Marcus E.M. Baumann; Michael Keusgen; Michael J. Schöning

doi:10.1002/pssa.201100801

Nutrient concentration-sensitive microorganism-based biosensor

Carl Frederik Werner, Simone Groebel, Christoph Krumbe, Torsten Wagner, Thorsten Selmer, Tatsuo Yoshinobu, Marcus E.M. Baumann, Michael Keusgen, Michael J. Schöning

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

This work presents a biosensor based on living cells immobilised on a light-addressable potentiometric sensor (LAPS). The set-up is sensitive to the concentrations of nutrients metabolised by bacteria, which results in an extracellular acidification. This is exemplarily demonstrated with glucose as nutrient and Escherichia coli as microorganism. The function of this biosensor is comparable to an enzyme-based field-effect sensor when using diffusion limitation. With the help of addressability of the LAPS an on-chip differential set-up was developed. Thus, external influences such as sensor drift, temperature and external pH changes can be minimised.

Original language	English
Pages (from-to)	900-904
Number of pages	5
Journal	Physica Status Solidi (A) Applications and Materials Science
Volume	209
Issue number	5
DOIs	https://doi.org/10.1002/pssa.201100801
Publication status	Published - 2012 May

Keywords

biosensor
extracellular acidification
glucose
light-addressable potentiometric sensor

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Surfaces and Interfaces
Surfaces, Coatings and Films
Electrical and Electronic Engineering
Materials Chemistry

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10.1002/pssa.201100801

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Nutrient concentration-sensitive microorganism-based biosensor. / Werner, Carl Frederik; Groebel, Simone; Krumbe, Christoph; Wagner, Torsten; Selmer, Thorsten; Yoshinobu, Tatsuo; Baumann, Marcus E.M.; Keusgen, Michael; Schöning, Michael J.

In: Physica Status Solidi (A) Applications and Materials Science, Vol. 209, No. 5, 05.2012, p. 900-904.

Research output: Contribution to journal › Article › peer-review

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