TY - JOUR
T1 - Numerical analysis of ultrasound propagation and reflection intensity for biological acoustic impedance microscope
AU - Gunawan, Agus Indra
AU - Hozumi, Naohiro
AU - Yoshida, Sachiko
AU - Saijo, Yoshifumi
AU - Kobayashi, Kazuto
AU - Yamamoto, Seiji
N1 - Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2015/8/1
Y1 - 2015/8/1
N2 - Abstract This paper proposes a new method for microscopic acoustic imaging that utilizes the cross sectional acoustic impedance of biological soft tissues. In the system, a focused acoustic beam with a wide band frequency of 30-100 MHz is transmitted across a plastic substrate on the rear side of which a soft tissue object is placed. By scanning the focal point along the surface, a 2-D reflection intensity profile is obtained. In the paper, interpretation of the signal intensity into a characteristic acoustic impedance is discussed. Because the acoustic beam is strongly focused, interpretation assuming vertical incidence may lead to significant error. To determine an accurate calibration curve, a numerical sound field analysis was performed. In these calculations, the reflection intensity from a target with an assumed acoustic impedance was compared with that from water, which was used as a reference material. The calibration curve was determined by changing the assumed acoustic impedance of the target material. The calibration curve was verified experimentally using saline solution, of which the acoustic impedance was known, as the target material. Finally, the cerebellar tissue of a rat was observed to create an acoustic impedance micro profile. In the paper, details of the numerical analysis and verification of the observation results will be described.
AB - Abstract This paper proposes a new method for microscopic acoustic imaging that utilizes the cross sectional acoustic impedance of biological soft tissues. In the system, a focused acoustic beam with a wide band frequency of 30-100 MHz is transmitted across a plastic substrate on the rear side of which a soft tissue object is placed. By scanning the focal point along the surface, a 2-D reflection intensity profile is obtained. In the paper, interpretation of the signal intensity into a characteristic acoustic impedance is discussed. Because the acoustic beam is strongly focused, interpretation assuming vertical incidence may lead to significant error. To determine an accurate calibration curve, a numerical sound field analysis was performed. In these calculations, the reflection intensity from a target with an assumed acoustic impedance was compared with that from water, which was used as a reference material. The calibration curve was determined by changing the assumed acoustic impedance of the target material. The calibration curve was verified experimentally using saline solution, of which the acoustic impedance was known, as the target material. Finally, the cerebellar tissue of a rat was observed to create an acoustic impedance micro profile. In the paper, details of the numerical analysis and verification of the observation results will be described.
KW - Acoustic impedance
KW - Plane wave
KW - Tissue
KW - Ultrasound
KW - k-space
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U2 - 10.1016/j.ultras.2015.03.010
DO - 10.1016/j.ultras.2015.03.010
M3 - Article
C2 - 25890637
AN - SCOPUS:84929606447
VL - 61
SP - 79
EP - 87
JO - Ultrasonics
JF - Ultrasonics
SN - 0041-624X
M1 - 5027
ER -