Nucleus accumbens associated 1 is recruited within the promyelocytic leukemia nuclear body through SUMO modification

Yoshinori Tatemichi, Masahiko Shibazaki, Shinji Yasuhira, Shuya Kasai, Hiroshi Tada, Hiroki Oikawa, Yuji Suzuki, Yasuhiro Takikawa, Tomoyuki Masuda, Chihaya Maesawa

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Nucleus accumbens associated 1 (NACC1) is a cancer-associated BTB/POZ (pox virus and zinc finger/bric-a-brac tramtrack broad complex) gene, and is involved in several cellular functions in neurons, cancer and stem cells. Some of the BTB/POZ proteins associated with cancer biology are SUMOylated, which appears to play an important role in transcription regulation. We show that NACC1 is SUMOylated on a phylogenetically conserved lysine (K167) out of three consensus SUMOylation motif sites. Amino acid substitution in the SIM sequence (SIM/M) within the BTB/POZ domain partially reduced K167 SUMOylation activity of NACC1. Overexpression of GFP-NACC1 fusion protein leads to formation of discrete nuclear foci similar to promyelocytic leukemia nuclear bodies (PML-NB), which colocalized with SUMO paralogues (SUMO1/2/3). Both NACC1 nuclear body formation and colocalization with SUMO paralogues were completely suppressed in the GFP-NACC1-SIM/M mutant, whereas they were partially maintained in the NACC1 K167R mutant. Confocal immunofluorescence analysis showed that endogenous and exogenous NACC1 proteins colocalized with endogenous PML protein. A pull-down assay revealed that the consensus motifs of the SUMO acceptor site at K167 and the SIM within the BTB/POZ domain were both necessary for efficient binding to PML protein. Our study demonstrates that NACC1 can be modified by SUMO paralogues, and cooperates with PML protein.

    Original languageEnglish
    Pages (from-to)848-856
    Number of pages9
    JournalCancer science
    Volume106
    Issue number7
    DOIs
    Publication statusPublished - 2015 Jul 1

    Keywords

    • Migration
    • Nucleus accumbens associated 1
    • Promyelocytic leukemia nuclear body
    • SUMO-interacting motif
    • SUMOylations

    ASJC Scopus subject areas

    • Oncology
    • Cancer Research

    Fingerprint Dive into the research topics of 'Nucleus accumbens associated 1 is recruited within the promyelocytic leukemia nuclear body through SUMO modification'. Together they form a unique fingerprint.

  • Cite this

    Tatemichi, Y., Shibazaki, M., Yasuhira, S., Kasai, S., Tada, H., Oikawa, H., Suzuki, Y., Takikawa, Y., Masuda, T., & Maesawa, C. (2015). Nucleus accumbens associated 1 is recruited within the promyelocytic leukemia nuclear body through SUMO modification. Cancer science, 106(7), 848-856. https://doi.org/10.1111/cas.12680