Nucleotide sequence of the tobacco mosaic virus (tomato strain) genome and comparison with the common strain genome

Takeshi Ohno, Midori Aoyagi, Yuji Yamanashi, Hiraku Saito, Shuntaro Ikawa, Tetsuo Meshi, Yoshimi Okada

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

The sequence of about 4,500 nucleotides of the internal part of tobacco mosaic virus (TMV)tomato strain (L) RNA has been newly determined using cloned cDNAs. Together with the previously determined partial sequences at both ends, the entire sequence of the 6,384 nucleotide genome has been completed. The 130K (1,115 amino acids), 180K (1,615 amino acids), 30K (263 amino acids) and coat protein (158 amino acids) cistrons are located at residues 72-3442, 72-4922, 4906-5700, and 5703-6182 on the genome, respectively. Sequence polymorphism was not observed except for heterogeneity in the length of the A cluster near the 3' end. The homology of the nucleotide sequences of TMV-L and TMV-vulgare, a common strain, is about 80%on average. Remarkable differences between them were found in a part of the N-terminal portion of the 130K/180K protein and the C-terminal portion of the 30K protein. A new method for cDNA cloning was developed by which the cDNA of the 5'-terminus of viral RNA can be cloned efficiently.

Original languageEnglish
Pages (from-to)1915-1923
Number of pages9
JournalJournal of biochemistry
Volume96
Issue number6
DOIs
Publication statusPublished - 1984 Oct
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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