Nucleotide sequence of the Thiobacillus ferrooxidans chromosomal gene encoding mercuric reductase

Chihiro Inoue, Kazuyuki Sugawara, Toshikazu Shiratori, Tomonobu Kusano, Yoshichika Kitagawa

    Research output: Contribution to journalArticle

    52 Citations (Scopus)

    Abstract

    The nucleotide sequence of the Thiobacillus ferrooxidans chromosomal mercuric-reductase-encoding gene (merA) has been determined. The merA gene contains 1635 bp, and shares 78.2% and 76.6% sequence homology with the transposon, Tn501, and plasmid R100 merA genes, respectively. From the sequence, a 545-amino acid (aa) polypeptide was deduced, and comparison with those of Tn501 and R100 revealed 80.6% and 80.0% homology, respectively, at the aa sequence level. Divergence among the three merA aa sequences was clustered within a specific region (aa positions 41-87). By analysis of codon usage frequency, it is speculated that the T. ferrooxidans merA gene originated from Tn501, R100, or a common ancestral gene, but not from T. ferrooxidans itself.

    Original languageEnglish
    Pages (from-to)47-54
    Number of pages8
    JournalGene
    Volume84
    Issue number1
    DOIs
    Publication statusPublished - 1989 Dec 7

    Keywords

    • Mercury resistance
    • codon usage
    • protein homology
    • recombinant DNA

    ASJC Scopus subject areas

    • Genetics

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