Nucleotide sequence of calf prorennin cDNA cloned in Escherichia coli

Katsuhiko Nishimori, Yoshiyuki Kawaguchi, Makoto Hidaka, Takeshi Uozumi, Teruhiko Beppu

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

The nucleotide sequence of prorennin (prochymosin) cDNA cloned in E. coli was determined by the technique of Maxam and Gilbert. The longest prorennin cDNA insert in pTACR1 contained the putative signal sequence and the coding sequence for the peptide from the 1st amino acid, Ala (NH2 terminal), to the 296th, Ser, and the other clone pTACR9 contained the coding sequence from the 258th, Asp, to the 365th, Ile (COOH terminal), and the TGA termination codon followed by the 3′-untranslated region. Thus, the whole coding sequence for prorennin was obtained in the pair of pTACR1 and pTACR9.

Original languageEnglish
Pages (from-to)1085-1088
Number of pages4
JournalJournal of biochemistry
Volume91
Issue number3
DOIs
Publication statusPublished - 1982 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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