Nucleotide sequence determination of mouse, chicken and Xenopuslaevisrig cDNAs: The rig-encoded protein is extremely conserved during vertebrate evolution

Akira Sugawa, Koji Nata, Chiyoko Inoue, Shin Takasawa, Hiroshi Yamamoto, Hiroshi Okamoto

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Mouse, chicken and Xenopuslaevis homologues to rig (rat insulinoma gene) cDNA were isolated and their nucleotide sequences were determined. Each homologue encoded a 145-amino acid protein; the amino acid sequence remained invariant in the murine and avian genes, and there were only 6 amino acid substitutions in the salientian gene. The evolutionary rate calculated for rig mRNA was sufficiently low to be viewed as evidence that rig is vital to vertebrate species. Southern blot analysis indicated that haploid sets of the mammalian genomes contain several copies of rig or rig-related sequences, whereas there appeared to be only one copy in the amphibian and bird genomes. The possibility that rig belongs to the class of housekeeping genes is discussed.

Original languageEnglish
Pages (from-to)1501-1507
Number of pages7
JournalBiochemical and biophysical research communications
Volume166
Issue number3
DOIs
Publication statusPublished - 1990 Feb 14

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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