Nucleotide sequence determination of chicken glucagon precursor cDNA. Chicken preproglucagon does not contain glucagon-like peptide II

Shin Hasegawa; Kimio Terazono; Koji Nata; Tatsuyuki Takada; Hiroshi Yamamoto; Hiroshi Okamoto

doi:10.1016/0014-5793(90)80779-I

Nucleotide sequence determination of chicken glucagon precursor cDNA. Chicken preproglucagon does not contain glucagon-like peptide II

Shin Hasegawa, Kimio Terazono, Koji Nata, Tatsuyuki Takada, Hiroshi Yamamoto, Hiroshi Okamoto

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Abstract

cDNA clones coding for glucagon were isolated from a chicken pancreas cDNA library, and the nucleotide and amino acid sequences were determined. The amino acid sequence of chicken glucagon was HSQGTFTSDYSKYLDSRRAQDFVQWLMST, which was contained in the 151-amino acid long precursor, being preceded by a signal sequence and an amino-terminal peptide (NH₂-peptide) and followed by an intervening peptide and a glucagon-like peptide I (GLP-I). Chicken preproglucagon, however, lacked GLP-II and intervening peptide II which have been shown to be contained in mammalian glucagon precursors.

Original language	English
Pages (from-to)	117-120
Number of pages	4
Journal	FEBS Letters
Volume	264
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(90)80779-I
Publication status	Published - 1990 May 7

Keywords

Gallus gallus domesticus
Glucagon
Glucagon-like peptide
Preproglucagon
cDNA cloning
mRNA

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(90)80779-I

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title = "Nucleotide sequence determination of chicken glucagon precursor cDNA. Chicken preproglucagon does not contain glucagon-like peptide II",

abstract = "cDNA clones coding for glucagon were isolated from a chicken pancreas cDNA library, and the nucleotide and amino acid sequences were determined. The amino acid sequence of chicken glucagon was HSQGTFTSDYSKYLDSRRAQDFVQWLMST, which was contained in the 151-amino acid long precursor, being preceded by a signal sequence and an amino-terminal peptide (NH2-peptide) and followed by an intervening peptide and a glucagon-like peptide I (GLP-I). Chicken preproglucagon, however, lacked GLP-II and intervening peptide II which have been shown to be contained in mammalian glucagon precursors.",

keywords = "Gallus gallus domesticus, Glucagon, Glucagon-like peptide, Preproglucagon, cDNA cloning, mRNA",

author = "Shin Hasegawa and Kimio Terazono and Koji Nata and Tatsuyuki Takada and Hiroshi Yamamoto and Hiroshi Okamoto",

note = "Funding Information: AcknowledgementTsh: is work hasb eens upportedin part by Grants-in-Aid for Scientific Researchf rom the Ministry of Education, Sciencea nd Culture, Japan.",

year = "1990",

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doi = "10.1016/0014-5793(90)80779-I",

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T1 - Nucleotide sequence determination of chicken glucagon precursor cDNA. Chicken preproglucagon does not contain glucagon-like peptide II

AU - Hasegawa, Shin

AU - Terazono, Kimio

AU - Nata, Koji

AU - Takada, Tatsuyuki

AU - Yamamoto, Hiroshi

AU - Okamoto, Hiroshi

N1 - Funding Information: AcknowledgementTsh: is work hasb eens upportedin part by Grants-in-Aid for Scientific Researchf rom the Ministry of Education, Sciencea nd Culture, Japan.

PY - 1990/5/7

Y1 - 1990/5/7

N2 - cDNA clones coding for glucagon were isolated from a chicken pancreas cDNA library, and the nucleotide and amino acid sequences were determined. The amino acid sequence of chicken glucagon was HSQGTFTSDYSKYLDSRRAQDFVQWLMST, which was contained in the 151-amino acid long precursor, being preceded by a signal sequence and an amino-terminal peptide (NH2-peptide) and followed by an intervening peptide and a glucagon-like peptide I (GLP-I). Chicken preproglucagon, however, lacked GLP-II and intervening peptide II which have been shown to be contained in mammalian glucagon precursors.

AB - cDNA clones coding for glucagon were isolated from a chicken pancreas cDNA library, and the nucleotide and amino acid sequences were determined. The amino acid sequence of chicken glucagon was HSQGTFTSDYSKYLDSRRAQDFVQWLMST, which was contained in the 151-amino acid long precursor, being preceded by a signal sequence and an amino-terminal peptide (NH2-peptide) and followed by an intervening peptide and a glucagon-like peptide I (GLP-I). Chicken preproglucagon, however, lacked GLP-II and intervening peptide II which have been shown to be contained in mammalian glucagon precursors.

KW - Gallus gallus domesticus

KW - Glucagon

KW - Glucagon-like peptide

KW - Preproglucagon

KW - cDNA cloning

KW - mRNA

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JF - FEBS Letters

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Nucleotide sequence determination of chicken glucagon precursor cDNA. Chicken preproglucagon does not contain glucagon-like peptide II

Abstract

Keywords

ASJC Scopus subject areas

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