Nucleotide sequence and expression of the glucoamylase-encoding gene (glaA) from Aspergillus oryzae

Hata Yoji, Tsuchiya Kozo, Kitamoto Katsuhiko, Gomi Katsuya, Kmnagai Chieko, Tamura Gakuzo, Hara Shodo

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

The glucoamylase-encoding gene (glaA) from Aspergillus oryzae was cloned using its cDNA as a probe, which had been isolated previously. From comparison of nucleotide (nt) sequences of genomic clones with its cDNA, the glaA gene was found to contain four short putative introns, 45-56 nt in length. The A. oryzae glaA gene shared 62% homology at the nt level with the A. niger glaA gene with the four introns located at the same position. The 5'-flanking region contained a TATA box at nt -72 from the start codon, and two putative CAAT sequences at nt -87 and -331. Genomic Southern analysis and physical mapping showed that the glaA gene is located on the smallest chromosome (3.4 Mb) of six separated bands of chromosomes. Clones containing the glaA gene, when re-introduced intro A. oryzae, resulted in a three- to eightfold increase in glucoamylase activity.

Original languageEnglish
Pages (from-to)145-150
Number of pages6
JournalGene
Volume108
Issue number1
DOIs
Publication statusPublished - 1991 Dec 1

Keywords

  • Ascomycetes
  • Recombinant DNA
  • gene structure
  • intron
  • physical mapping
  • sake
  • transformation

ASJC Scopus subject areas

  • Genetics

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