Nucleotide sequence analysis of S-locus genes to unify S haplotype nomenclature in radish

Yurie Haseyama, Hiroyasu Kitashiba, Shunsuke Okamoto, Emiko Tonouchi, Koji Sakamoto, Takeshi Nishio

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Radish, belonging to the family Brassicaceae, has a self-incompatibility which is controlled by multiple alleles on the S locus. To employ the self-incompatibility in an F1 breeding system, identification of S haplotypes is necessary. Since collection of S haplotypes and determination of nucleotide sequences of SLG, SRK, and SCR alleles in cultivated radish have been conducted by different groups independently, the same or similar sequences with different S haplotype names and different sequences with the same S haplotype names have been registered in public databases, resulting in confusion of S haplotype names for researchers and breeders. In the present study, we developed S homozygous lines from radish F1 hybrid cultivars in Japan and determined the nucleotide sequences of SCR, the S domain and the kinase domain of SRK, and the SLG of a large number of S haplotypes. Comparing these sequences with our previously published sequences, the haplotypes were ordered into 23 different S haplotypes. The sequences of the 23 S haplotypes were compared with S haplotype sequences registered by different groups, and we suggested a unification of these S haplotypes. Furthermore, dot-blot hybridization using SRK allele-specific probes was examined for developing a standard method for S haplotype identification.

Original languageEnglish
Article number116
JournalMolecular Breeding
Volume38
Issue number9
DOIs
Publication statusPublished - 2018 Sep 1

Keywords

  • Radish
  • S haplotype identification
  • S haplotype nomenclature
  • Self-incompatibility

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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