Nucleosomal Structure of Marek's Disease Virus Genome In Transformed Lymphoblastoid Cell Lines, Mdcc-msb1 and Mdcc-rp1

The latent MDV (Marek’s disease virus) genomes are folded into nucleo-somal structures in both virus-productive and -nonproductive lymphoblastoid cell lines, MDCC-MSB1 (MSB-1) and -RP1 (RP-1), respectively. There was no difference between transcriptionally active and inactive regions of MDV genome with regard to nucleosomal patterns. In order to investigate whether nucleosomal structure is correlated with the repression of the transcription of MDV genome in lymphoblastoid cells, we examined the DNase I sensitivity of nucleosomal MDV DNA in lymphoblastoid cell lines, MSB-1 and RP-1. No difference in the DNasel sensitivity between transcriptitious active and inactive MDV DNA regions in lympho-blastoid cells was observed.