TY - JOUR
T1 - Nuclear Transfer in Cattle
T2 - Effect of Linoleic Acid-Albumin on Freezing Sensitivity of Enucleated Oocytes
AU - Hochi, Shinichi
AU - Kato, Megumi
AU - Ito, Kazumi
AU - Hirabayashi, Masumi
AU - Ueda, Masatsugu
AU - Sekimoto, Akiyo
AU - Nagao, Yoshikazu
AU - Kimura, Ken
AU - Hanada, Akira
PY - 2000/10
Y1 - 2000/10
N2 - The effect of linoleic acid-albumin (LAA) supplementation to the media for IVM, enucleation, and activation on the developmental potential of bovine embryos produced by nuclear transfer (NT) into frozen-thawed cytoplasts was investigated. Blastomeres derived from morulae was placed in the perivitelline space of frozen-thawed cytoplasts, which were then fused by a DC pulse. The proportion of fused embryos was similar between groups with and without LAA (87 vs. 90%). The proportion of development to blastocysts of NT embryos derived from the media with LAA (14%) was higher than that without LAA (4%), indicating that LAA treatment of bovine oocytes during IVM, enucleation and activation can improve the ability of such cytoplasts after freezing and thawing to develop into blastocysts after NT.
AB - The effect of linoleic acid-albumin (LAA) supplementation to the media for IVM, enucleation, and activation on the developmental potential of bovine embryos produced by nuclear transfer (NT) into frozen-thawed cytoplasts was investigated. Blastomeres derived from morulae was placed in the perivitelline space of frozen-thawed cytoplasts, which were then fused by a DC pulse. The proportion of fused embryos was similar between groups with and without LAA (87 vs. 90%). The proportion of development to blastocysts of NT embryos derived from the media with LAA (14%) was higher than that without LAA (4%), indicating that LAA treatment of bovine oocytes during IVM, enucleation and activation can improve the ability of such cytoplasts after freezing and thawing to develop into blastocysts after NT.
KW - Cloning
KW - Cryopreservation
KW - Linoleic acid-albumin
UR - http://www.scopus.com/inward/record.url?scp=0034304532&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034304532&partnerID=8YFLogxK
U2 - 10.1292/jvms.62.1111
DO - 10.1292/jvms.62.1111
M3 - Article
C2 - 11073086
AN - SCOPUS:0034304532
VL - 62
SP - 1111
EP - 1113
JO - Journal of Veterinary Medical Science
JF - Journal of Veterinary Medical Science
SN - 0916-7250
IS - 10
ER -