Nuclear localization of sweet cherry DREB1/CBF ortholog (CIG) and low temperature-inducible activity of CIG promoter

Hiroyasu Kitashiba, Yusuke Ban, Chikako Honda, Takaya Moriguchi

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The fluorescent signal of GFP fused to CIG-B, which is one of the three DREB1/CBF (dehydration responsive element binding protein 1/C-repeat binding factor) homologs isolated from sweet cherry, was observed only in nucleus. The expression of GFP driven by CIG-B promoter was up-regulated by low-temperature. These results suggested that nuclear localization signal (NLS) motif in the N-terminal region of CIG-B protein and inducer of CBF expression (ICE)-like motifs in CIG-B promoter could be responsible for nuclear targeting and low temperature perception, respectively.

Original languageEnglish
Pages (from-to)515-518
Number of pages4
JournalPlant Biotechnology
Volume23
Issue number5
DOIs
Publication statusPublished - 2006

Keywords

  • DREB/CBF
  • Green fluorescence protein
  • Nuclear localization signal
  • Sweet cherry

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

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