Nuclear localization and involvement in DNA synthesis of Sarcophaga prolyl endopeptidase

Sumio Ohtsuki, Ko Ichi Homma, Shoichiro Kurata, Shunji Natori

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

A specific prolyl endopeptidase (PEP) inhibitor, ZTTA, selectively inhibited DNA synthesis by imaginal discs and cultured embryonic cells of Sarcophaga peregrina (flesh fly). PEP was found to be localized in restricted nuclear regions. Unfertilized eggs were shown to contain a maternal message for PEP and analysis of Sarcophaga embryos at blastdermal stage revealed PEP was localized exclusively in the nuclei. These results suggest that PEP participates in DNA synthesis by, and therefore cell proliferation, of insect cells. This is the first demonstration of a biological function of PEP.

Original languageEnglish
Pages (from-to)1176-1181
Number of pages6
JournalJournal of biochemistry
Volume121
Issue number6
DOIs
Publication statusPublished - 1997 Jun
Externally publishedYes

Keywords

  • DNA synthesis
  • Imaginal disc
  • Nuclear localization
  • Prolyl endopeptidase
  • Sarcophaga peregrina

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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