Nuclear localization and involvement in DNA synthesis of Sarcophaga prolyl endopeptidase

Sumio Ohtsuki; Ko Ichi Homma; Shoichiro Kurata; Shunji Natori

doi:10.1093/oxfordjournals.jbchem.a021712

Nuclear localization and involvement in DNA synthesis of Sarcophaga prolyl endopeptidase

Sumio Ohtsuki, Ko Ichi Homma, Shoichiro Kurata, Shunji Natori

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

A specific prolyl endopeptidase (PEP) inhibitor, ZTTA, selectively inhibited DNA synthesis by imaginal discs and cultured embryonic cells of Sarcophaga peregrina (flesh fly). PEP was found to be localized in restricted nuclear regions. Unfertilized eggs were shown to contain a maternal message for PEP and analysis of Sarcophaga embryos at blastdermal stage revealed PEP was localized exclusively in the nuclei. These results suggest that PEP participates in DNA synthesis by, and therefore cell proliferation, of insect cells. This is the first demonstration of a biological function of PEP.

Original language	English
Pages (from-to)	1176-1181
Number of pages	6
Journal	Journal of biochemistry
Volume	121
Issue number	6
DOIs	https://doi.org/10.1093/oxfordjournals.jbchem.a021712
Publication status	Published - 1997 Jun
Externally published	Yes

Keywords

DNA synthesis
Imaginal disc
Nuclear localization
Prolyl endopeptidase
Sarcophaga peregrina

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1093/oxfordjournals.jbchem.a021712

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abstract = "A specific prolyl endopeptidase (PEP) inhibitor, ZTTA, selectively inhibited DNA synthesis by imaginal discs and cultured embryonic cells of Sarcophaga peregrina (flesh fly). PEP was found to be localized in restricted nuclear regions. Unfertilized eggs were shown to contain a maternal message for PEP and analysis of Sarcophaga embryos at blastdermal stage revealed PEP was localized exclusively in the nuclei. These results suggest that PEP participates in DNA synthesis by, and therefore cell proliferation, of insect cells. This is the first demonstration of a biological function of PEP.",

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author = "Sumio Ohtsuki and Homma, {Ko Ichi} and Shoichiro Kurata and Shunji Natori",

year = "1997",

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T1 - Nuclear localization and involvement in DNA synthesis of Sarcophaga prolyl endopeptidase

AU - Ohtsuki, Sumio

AU - Homma, Ko Ichi

AU - Kurata, Shoichiro

AU - Natori, Shunji

PY - 1997/6

Y1 - 1997/6

N2 - A specific prolyl endopeptidase (PEP) inhibitor, ZTTA, selectively inhibited DNA synthesis by imaginal discs and cultured embryonic cells of Sarcophaga peregrina (flesh fly). PEP was found to be localized in restricted nuclear regions. Unfertilized eggs were shown to contain a maternal message for PEP and analysis of Sarcophaga embryos at blastdermal stage revealed PEP was localized exclusively in the nuclei. These results suggest that PEP participates in DNA synthesis by, and therefore cell proliferation, of insect cells. This is the first demonstration of a biological function of PEP.

AB - A specific prolyl endopeptidase (PEP) inhibitor, ZTTA, selectively inhibited DNA synthesis by imaginal discs and cultured embryonic cells of Sarcophaga peregrina (flesh fly). PEP was found to be localized in restricted nuclear regions. Unfertilized eggs were shown to contain a maternal message for PEP and analysis of Sarcophaga embryos at blastdermal stage revealed PEP was localized exclusively in the nuclei. These results suggest that PEP participates in DNA synthesis by, and therefore cell proliferation, of insect cells. This is the first demonstration of a biological function of PEP.

KW - DNA synthesis

KW - Imaginal disc

KW - Nuclear localization

KW - Prolyl endopeptidase

KW - Sarcophaga peregrina

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Nuclear localization and involvement in DNA synthesis of Sarcophaga prolyl endopeptidase

Abstract

Keywords

ASJC Scopus subject areas

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