NtbZIP60, an endoplasmic reticulum-localized transcription factor, plays a role in the defense response against bacterial pathogens in Nicotiana tabacum

Chika Tateda, Rei Ozaki, Yu Onodera, Yoshihiro Takahashi, Koji Yamaguchi, Thomas Berberich, Nozomu Koizumi, Tomonobu Kusano

Research output: Contribution to journalArticlepeer-review

49 Citations (Scopus)

Abstract

A spermine-based signal transduction pathway plays a defensive role against incompatible pathogens. We identified a novel spermine-responsive cDNA from Nicotiana tabacum that encodes a basic region/leucine zipper protein with a putative transmembrane domain. Identity to Arabidopsis thaliana AtbZIP60 was sufficiently high to name the novel cDNA NtbZIP60. Expression analysis revealed that NtbZIP60 is a component of the spermine-signal pathway, and is also involved in the unfolded protein response (UPR), as demonstrated for AtbZIP60. The gene product, NtbZIP60, localizes to the endoplasmic reticulum (ER) in plant cells; once the putative transmembrane domain is eliminated from the intact protein, it targets the nucleus. The putative processed form of NtbZIP60 transactivates target genes through binding to plant-specific UPR cis-elements. Expression of NbbZIP60, an NtbZIP60 ortholog in Nicotiana benthamiana, was significantly up-regulated at 6 h and later time points upon infection with the non-host pathogen Pseudomonas cichorii, while it was unaffected by infection with the compatible pathogen Pseudomonas syringae pv. tabaci. Furthermore, NbbZIP60-silenced N. benthamiana plants allowed higher multiplication of P. cichorii compared to the control plants. Taken together, the results suggest that this ER-localized transcription factor is involved in the spermine-signal transduction pathway and plays an important role in plant innate immunity.

Original languageEnglish
Pages (from-to)603-611
Number of pages9
JournalJournal of Plant Research
Volume121
Issue number6
DOIs
Publication statusPublished - 2008 Nov 1

Keywords

  • Bacterial pathogen
  • Defense
  • Endoplasmic reticulum
  • Spermine
  • Transcription factor

ASJC Scopus subject areas

  • Plant Science

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