NRF2 and HSF1 coordinately regulate heme oxygenase-1 expression

Sachiye Inouye, Yuta Hatori, Takanori Kubo, Shizuka Saito, Hiroshi Kitamura, Reiko Akagi

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


Heme oxygenase-1 (HO-1) is an inducible enzyme responding to various stresses and has cytoprotective activities. Although HO-1 has been referred to as heat shock protein (HSP) 32, the heat-mediated induction of HO-1 varies among different species and cell lines. We examined the effects of heat shock on HO-1 expression in mouse embryonic fibroblast (MEF) cells deficient in heat shock factor 1 (HSF1) or nuclear factor-erythroid-2-related factor 2 (NRF2). Heme-induced expression of HO-1 was 2-fold higher in Hsf1−/− cells than in the wild-type cells at both mRNA and protein levels. In Nrf2−/− cells, heme-induced expression of HO-1 was not detected. In contrast, HO-1 expression was markedly induced by heat shock at 40–42 °C in Nrf2−/− cells while the wild-type cells were not responsive. The heat-induced expression of HO-1 in Nrf2−/− cells were almost completely diminished by transfection of siRNA against Hsf1 gene. These results suggest that HSF1 and NRF2 suppress heme-induced and heat-induced HO-1 expression, respectively.

Original languageEnglish
Pages (from-to)7-11
Number of pages5
JournalBiochemical and biophysical research communications
Issue number1
Publication statusPublished - 2018 Nov 17


  • Heat shock factor 1 (HSF1)
  • Heme oxygenase-1 (HO-1)
  • Nuclear factor-erythroid-2-related factor 2 (NRF2)
  • Stress response

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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