TY - JOUR
T1 - NRF2 and HSF1 coordinately regulate heme oxygenase-1 expression
AU - Inouye, Sachiye
AU - Hatori, Yuta
AU - Kubo, Takanori
AU - Saito, Shizuka
AU - Kitamura, Hiroshi
AU - Akagi, Reiko
N1 - Funding Information:
We are grateful to Dr. Hozumi Motohashi (Tohoku University) and Dr. Akira Nakai (Yamaguchi University) for suggestion. This work was supported in part by Grants-in- Aid for Scientific Research from Ministry of Education, Science, and Culture of Japan and a grant from Yasuda Women's University.
Funding Information:
We are grateful to Dr. Hozumi Motohashi (Tohoku University) and Dr. Akira Nakai (Yamaguchi University) for suggestion. This work was supported in part by Grants-in- Aid for Scientific Research from Ministry of Education, Science, and Culture of Japan and a grant from Yasuda Women’s University .
Publisher Copyright:
© 2018 Elsevier Inc.
PY - 2018/11/17
Y1 - 2018/11/17
N2 - Heme oxygenase-1 (HO-1) is an inducible enzyme responding to various stresses and has cytoprotective activities. Although HO-1 has been referred to as heat shock protein (HSP) 32, the heat-mediated induction of HO-1 varies among different species and cell lines. We examined the effects of heat shock on HO-1 expression in mouse embryonic fibroblast (MEF) cells deficient in heat shock factor 1 (HSF1) or nuclear factor-erythroid-2-related factor 2 (NRF2). Heme-induced expression of HO-1 was 2-fold higher in Hsf1−/− cells than in the wild-type cells at both mRNA and protein levels. In Nrf2−/− cells, heme-induced expression of HO-1 was not detected. In contrast, HO-1 expression was markedly induced by heat shock at 40–42 °C in Nrf2−/− cells while the wild-type cells were not responsive. The heat-induced expression of HO-1 in Nrf2−/− cells were almost completely diminished by transfection of siRNA against Hsf1 gene. These results suggest that HSF1 and NRF2 suppress heme-induced and heat-induced HO-1 expression, respectively.
AB - Heme oxygenase-1 (HO-1) is an inducible enzyme responding to various stresses and has cytoprotective activities. Although HO-1 has been referred to as heat shock protein (HSP) 32, the heat-mediated induction of HO-1 varies among different species and cell lines. We examined the effects of heat shock on HO-1 expression in mouse embryonic fibroblast (MEF) cells deficient in heat shock factor 1 (HSF1) or nuclear factor-erythroid-2-related factor 2 (NRF2). Heme-induced expression of HO-1 was 2-fold higher in Hsf1−/− cells than in the wild-type cells at both mRNA and protein levels. In Nrf2−/− cells, heme-induced expression of HO-1 was not detected. In contrast, HO-1 expression was markedly induced by heat shock at 40–42 °C in Nrf2−/− cells while the wild-type cells were not responsive. The heat-induced expression of HO-1 in Nrf2−/− cells were almost completely diminished by transfection of siRNA against Hsf1 gene. These results suggest that HSF1 and NRF2 suppress heme-induced and heat-induced HO-1 expression, respectively.
KW - Heat shock factor 1 (HSF1)
KW - Heme oxygenase-1 (HO-1)
KW - Nuclear factor-erythroid-2-related factor 2 (NRF2)
KW - Stress response
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U2 - 10.1016/j.bbrc.2018.10.030
DO - 10.1016/j.bbrc.2018.10.030
M3 - Article
C2 - 30316516
AN - SCOPUS:85054597638
VL - 506
SP - 7
EP - 11
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -