Abstract
We have identified novel transcriptional isoforms of the human and mouse genes encoding muscle type phosphofructokinase-1 (PFK-M). These isoforms are expressed specifically in the testis and in the mid-gestation embryo, and have been termed TE-PFK-M (testis- and embryo-specific PFK-M). The 5′UTR of TE-PFK-M is composed of three newly identified exons that lie much farther upstream of the PFK-M coding region than the previously characterized 5′UTR. In addition, this upstream region encodes a series of small polyadenylated transcripts, some of which share the same exons found in the 5′UTR of TE-PFK-M, and which may play some role in regulating TE-PFK-M expression. These findings indicate an even more complex level of control of PFK-M expression than previously thought.
Original language | English |
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Pages (from-to) | 580-587 |
Number of pages | 8 |
Journal | Biochemical and biophysical research communications |
Volume | 316 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2004 Apr 2 |
Externally published | Yes |
Keywords
- 5 UTR
- Embryo
- Gene expression
- PFK-M
- Phosphofructokinase-1, muscle type
- Testis
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology