Novel PCR-mediated mutagenesis employing DNA containing a natural abasic site as a template and translesional Taq DNA polymerase

Atsushi Kobayashi, Motomitsu Kitaoka, Kiyoshi Hayashi

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

We describe a novel method of PCR-mediated mutagenesis employing DNA containing a natural abasic site and translesional Taq DNA polymerase. This method incorporated an adenine (80.8%) or guanine (7.7%) residue or led to a base deletion mutation (11.2%) opposite the abasic site. We conclude that the combination of DNA containing an abasic site and translesional Taq DNA polymerase is an easy and useful technique for PCR-mediated mutagenesis, having advantages different from those of conventional error-prone PCR.

Original languageEnglish
Pages (from-to)227-232
Number of pages6
JournalJournal of Biotechnology
Volume116
Issue number3
DOIs
Publication statusPublished - 2005 Mar 30

Keywords

  • Abasic site
  • Base-selective substitution mutagenesis
  • PCR-mediated mutagenesis
  • Translesional DNA polymerase

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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