We describe a novel method of PCR-mediated mutagenesis employing DNA containing a natural abasic site and translesional Taq DNA polymerase. This method incorporated an adenine (80.8%) or guanine (7.7%) residue or led to a base deletion mutation (11.2%) opposite the abasic site. We conclude that the combination of DNA containing an abasic site and translesional Taq DNA polymerase is an easy and useful technique for PCR-mediated mutagenesis, having advantages different from those of conventional error-prone PCR.
- Abasic site
- Base-selective substitution mutagenesis
- PCR-mediated mutagenesis
- Translesional DNA polymerase
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology