Novel methylcellulose-immobilized cation-exchange precolumn for on-line enrichment of cationic drugs in plasma

Eiichi Yamamoto, Takahisa Sakaguchi, Takashi Kajima, Nariyasu Mano, Naoki Asakawa

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

We developed a novel methylcellulose-immobilized strong cation-exchange (MC-SCX) precolumn for direct analysis of drugs in plasma. MC-SCX consists of silica gel with a methylcellulose outer-surface and a 2-(4-sulfophenyl) ethyl phase inner-surface. The MC-SCX precolumn was evaluated by direct analysis using pyridoxine, atenolol and sulpiride spiked in plasma, using a column-switching HPLC system. Each drug was retained and enriched on MC-SCX using an acidic mobile phase, which resulted in good linearity, sufficient reproducibility, intra- and inter day precision, and accuracy in analytical ion-pair LC with trifluoroacetic acid. The analytical methods for model drugs were applied to pharmacokinetics of atenolol and sulpiride in rats.

Original languageEnglish
Pages (from-to)327-334
Number of pages8
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume807
Issue number2
DOIs
Publication statusPublished - 2004 Aug 5

Keywords

  • Atenolol
  • Pharmacokinetics
  • Pyridoxine
  • Sulpiride

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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