TY - JOUR
T1 - Novel Method for Polysaccharide Synthesis Using an Enzyme
T2 - The First in Vitro Synthesis of Cellulose via a Nonbiosynthetic Path Utilizing Cellulase as Catalyst
AU - Kobayashi, Shiro
AU - Kashiwa, Keita
AU - Kawasaki, Tatsuya
AU - Shoda, Shin Ichiro
PY - 1991/1/1
Y1 - 1991/1/1
N2 - The in vitro synthesis of cellulose via a nonbiosynthetic path has been achieved for the first time by condensation of β-D-cellobiosyl fluoride as substrate for cellulase, a hydrolysis enzyme of cellulose, in a mixed solvent of acetonitrile/acetate buffer (pH 5, 5:1). The water-insoluble part of the products is “synthetic cellulose”, the structure of which was confirmed by comparison with an authentic natural cellulose sample with use of solid C NMR and IR spectroscopies as well as with a hydrolysis experiment. The present synthetic cellulose was converted to the corresponding triacetate whose molecular weight was at least 6.3 × 103 (degree of polymerization (DP) ≥ 22). X-ray as well as 13C NMR analyses showed that its crystal structure is of type II with high crystallinity. Under reaction conditions of a higher substrate concentration or higher acetonitrile concentration, water-soluble cellooligosaccharides (DP ≤ 8) were produced predominantly.
AB - The in vitro synthesis of cellulose via a nonbiosynthetic path has been achieved for the first time by condensation of β-D-cellobiosyl fluoride as substrate for cellulase, a hydrolysis enzyme of cellulose, in a mixed solvent of acetonitrile/acetate buffer (pH 5, 5:1). The water-insoluble part of the products is “synthetic cellulose”, the structure of which was confirmed by comparison with an authentic natural cellulose sample with use of solid C NMR and IR spectroscopies as well as with a hydrolysis experiment. The present synthetic cellulose was converted to the corresponding triacetate whose molecular weight was at least 6.3 × 103 (degree of polymerization (DP) ≥ 22). X-ray as well as 13C NMR analyses showed that its crystal structure is of type II with high crystallinity. Under reaction conditions of a higher substrate concentration or higher acetonitrile concentration, water-soluble cellooligosaccharides (DP ≤ 8) were produced predominantly.
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U2 - 10.1021/ja00008a042
DO - 10.1021/ja00008a042
M3 - Article
AN - SCOPUS:0001547441
VL - 113
SP - 3079
EP - 3084
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
SN - 0002-7863
IS - 8
ER -