Novel method for mass producing genetically sterile fish from surrogate broodstock via spermatogonial transplantation

Kazue Nagasawa, Mariko Ishida, Anna Octavera, Kazunari Kusano, Fumi Kezuka, Takeshi Kitano, Yasutoshi Yoshiura, Goro Yoshizaki

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

A stable system for producing sterile domesticated fish is required to prevent genetic contamination to native populations caused by aquaculture escapees. The objective of this study was to develop a system to mass produce stock for aquaculture that is genetically sterile by surrogate broodstock via spermatogonial transplantation (SGTP). We previously discovered that female medaka carrying mutations on the follicle-stimulating hormone receptor (fshr) gene become sterile. In this study, we demonstrated that sterile hybrid recipient females that received spermatogonia isolated from sex-reversed XX males (fshr (-/-)) recovered their fertility and produced only donor-derived fshr (-) X eggs. Natural mating between these females and fshr (-/-) sex-reversed XX males successfully produced large numbers of sterile fshr (-/-) female offspring. In conclusion, we established a new strategy for efficient mass production of sterile fish. This system can be applied to any aquaculture species for which SGTP and methods for producing sterile recipients can be established.

Original languageEnglish
Pages (from-to)535-546
Number of pages12
JournalBiology of Reproduction
Volume100
Issue number2
DOIs
Publication statusPublished - 2019 Feb 1
Externally publishedYes

Keywords

  • fshr mutants
  • germline stem cells
  • spermatogonial transplantation
  • sterile fish production

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology

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