Novel laccases of Ganoderma sp. KU-Alk4, regulated by different glucose concentration in alkaline media

Churapa Teerapatsakul, Naoki Abe, Christopher Bucke, Ngampong Kongkathip, Saeree Jareonkitmongkol, Lerluck Chitradon

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

Physiological regulation of laccase production from Ganoderma sp. KU-Alk4, isolated in Thailand, was controlled by the initial glucose concentration in liquid culture. Different laccase isozymes were produced using different starting concentrations of glucose. With 1% glucose, two isozymes, KULac 1 and 2 were produced, while with 4% glucose, three different isozymes, KULac 3, 4 and 5, were produced. The KULacs differed in their molecular mass, ranging from 53 to 112 kDa. KULac 2 was a new laccase that had a different N-terminal amino acid sequence from other laccases previously reported. All the isozymes had optimum pH at 3.5 and were stable over the wide range of pH, 3.0-10.0, especially in alkaline pH. It is noteworthy that the activities of the four KULacs with 2,6-dimethoxyphenol were extremely high up to 90°C. They retained 100% of their activities at 60°C for 1 h.

Original languageEnglish
Pages (from-to)1559-1567
Number of pages9
JournalWorld Journal of Microbiology and Biotechnology
Volume23
Issue number11
DOIs
Publication statusPublished - 2007 Nov

Keywords

  • Ganoderma
  • Glucose effect
  • Isozyme
  • Laccase
  • Purification

ASJC Scopus subject areas

  • Biotechnology
  • Physiology
  • Applied Microbiology and Biotechnology

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