Novel isoprenoid modified proteins in halobacteria

Hiroshi Sagami, Akihiro Kikuchi, Kyozo Ogura, Kazuhisa Fushihara, Tokuzo Nishino

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Incorporation of [3H]mevalonic acid-derived materials into proteins was studied with extremely halophilic archaebacteria, Halobacterium halobium and Halobacterium cutirubrum. Several labeled proteins were detected on SDS-polyacrylamide gel electrophoresis followed by fluorography. The majority of the radioactive materials released from the labeled proteins by sulfonium salt cleavage moved with a mobility similar to that of a C85 polyprenol on reverse-phase thin-layer chromatography, and no radioactive farnesol was found on the chromatography. However, a weak but significant protein farnesyltransferase activity was detected in in vitro experiments with a combination of [3H]farnesyl diphosphate and Ras precursor protein.

Original languageEnglish
Pages (from-to)972-978
Number of pages7
JournalBiochemical and biophysical research communications
Volume203
Issue number2
DOIs
Publication statusPublished - 1994 Sep 15

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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