Novel function of metallothionein in photoprotection: Metallothionein- null mouse exhibits reduced tolerance against ultraviolet B injury in the skin

Katsumi Hanada, Daisuke Sawamura, Katsuto Tamai, Takako Baba, Isao Hashimoto, Tsutomu Muramatsu, Nobuhiko Miura, Akira Naganuma

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

We have shown previously that injection of cadmium chloride (Cd2+) depletes the number of ultraviolet B (UVB)-induced sunburn cells in the mouse skin in vivo, and that Cd2+ treatment enhances UVB resistance in cultured keratinocytes in vitro, indicating the photoprotective role of Cd2+- induced metallothioneins (MT) with antioxidant property against UVB injury; however, there has been no direct evidence for the role of MT in UV protection. To improve our understanding of MT in photoprotection, MT-null mouse deficient in its MT-1 and MT-2 genes was studied. Skin explants were preliminarily exposed to medium alone, Cd2+ and Cd2+ plus buthionine S,R- sulfoximine, an inhibitor of glutathione synthesis. We then compared the number of UVB-induced sunburn cells and apoptotic cells in the epidermis of MT-null mice with that of control mice using organ culture systems. The skin of MT-null mice developed a greater number of sunburn cells and apoptotic cells than did that of normal mice in all experimental conditions. These findings indicate that the skin of MT-null mouse is readily injured by UVB irradiation. MT-null mouse provided direct evidence of the photoprotective effect of cellular MT in the skin.

Original languageEnglish
Pages (from-to)582-585
Number of pages4
JournalJournal of Investigative Dermatology
Volume111
Issue number4
DOIs
Publication statusPublished - 1998 Jan 1

Keywords

  • Apoptosis
  • Knockout mouse
  • Sunburn cell

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

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