Novel derivatization reagent with tetrathiafulvalene as an electrophore for pre-column labeling of amines in high-performance liquid chromatography

Kazutake Shimada, Tomoyuki Oe

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A novel derivatization reagent with tetrathiafulvalene as an electrophore, N-succinimidyl tetrathiafulvalene-2-carboxylate, was prepared and evaluated for pre-column labeling of amine by using 13-phenethylamine as a model compound in high-performance liquid chromatography with electrochemical detection. The reagent reacted readily with the primary amine at room temperature for 1 h, providing the amide which was highly responsive to an electrochemical detector (detection limit = 21 fmol, signal to noise ratio = 5) at a relatively low applied potential (+700 mV vs. Ag/AgC1). The characteristic two-stage oxidative pattern appeared on the hydrodynamic voltammogram of the derivative, which helped to identify the peak on the chromatogram.

Original languageEnglish
Pages (from-to)1897-1898
Number of pages2
JournalChemical and Pharmaceutical Bulletin
Volume39
Issue number7
DOIs
Publication statusPublished - 1991 Jan 1
Externally publishedYes

Keywords

  • N-succinimidyl tetrathiafulvalene-2-carboxylate
  • amine
  • electrochemical detection
  • electrophore
  • high-performance liquid chromatography
  • pre-column labeling
  • tetrathiafulvalene

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

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