A novel derivatization reagent with tetrathiafulvalene as an electrophore, N-succinimidyl tetrathiafulvalene-2-carboxylate, was prepared and evaluated for pre-column labeling of amine by using 13-phenethylamine as a model compound in high-performance liquid chromatography with electrochemical detection. The reagent reacted readily with the primary amine at room temperature for 1 h, providing the amide which was highly responsive to an electrochemical detector (detection limit = 21 fmol, signal to noise ratio = 5) at a relatively low applied potential (+700 mV vs. Ag/AgC1). The characteristic two-stage oxidative pattern appeared on the hydrodynamic voltammogram of the derivative, which helped to identify the peak on the chromatogram.
- N-succinimidyl tetrathiafulvalene-2-carboxylate
- electrochemical detection
- high-performance liquid chromatography
- pre-column labeling
ASJC Scopus subject areas
- Drug Discovery