TY - JOUR
T1 - Novel arbekacin- and amikacin-modifying enzyme of methicillin-resistant Staphylococcus aureus
AU - Fujimura, Shigeru
AU - Tokue, Yutaka
AU - Takahashi, Hiroshi
AU - Kobayashi, Takao
AU - Gomi, Kazunori
AU - Abe, Tatsuya
AU - Nukiwa, Toshihiro
AU - Watanabe, Akira
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 2000/9/15
Y1 - 2000/9/15
N2 - An aminoglycoside-modifying enzyme in arbekacin-resistant methicillin- resistant Staphylococcus aureus (MRSA), exhibiting 4'''-N-acetylation, was examined. Although the MRSA strain with AAC(4''') had no AAC(6')-APH(2'') activity, a DNA fragment of the AAC(6')-APH(2'') gene was amplified by PCR and the purified N-terminal 30-amino acid sequence of this AAC(4''') was identical to AAC(6')-APH(2''). Direct DNA sequencing of this 'silent' AAC(6')-APH(2'') gene revealed a single point mutation leading to a substitution of Gly for Asp80, through which the secondary structure is affected. A change in protein conformation could lead to a cleavage and a change of the enzymatic activity. We propose a new aminoglycoside-resistance mediated by AAC(4''') is caused by a mutation-modified AAC(6')-APH(2''). (C) 2000 Federation of European Microbiological Societies.
AB - An aminoglycoside-modifying enzyme in arbekacin-resistant methicillin- resistant Staphylococcus aureus (MRSA), exhibiting 4'''-N-acetylation, was examined. Although the MRSA strain with AAC(4''') had no AAC(6')-APH(2'') activity, a DNA fragment of the AAC(6')-APH(2'') gene was amplified by PCR and the purified N-terminal 30-amino acid sequence of this AAC(4''') was identical to AAC(6')-APH(2''). Direct DNA sequencing of this 'silent' AAC(6')-APH(2'') gene revealed a single point mutation leading to a substitution of Gly for Asp80, through which the secondary structure is affected. A change in protein conformation could lead to a cleavage and a change of the enzymatic activity. We propose a new aminoglycoside-resistance mediated by AAC(4''') is caused by a mutation-modified AAC(6')-APH(2''). (C) 2000 Federation of European Microbiological Societies.
KW - Amikacin
KW - Aminoglycoside acetyltransferase
KW - Aminoglycoside resistance
KW - Arbekacin
KW - Methicillin-resistant Staphylococcus aureus
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U2 - 10.1016/S0378-1097(00)00348-7
DO - 10.1016/S0378-1097(00)00348-7
M3 - Article
C2 - 11034295
AN - SCOPUS:0034666559
VL - 190
SP - 299
EP - 303
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 2
ER -