Novel arbekacin- and amikacin-modifying enzyme of methicillin-resistant Staphylococcus aureus

Shigeru Fujimura, Yutaka Tokue, Hiroshi Takahashi, Takao Kobayashi, Kazunori Gomi, Tatsuya Abe, Toshihiro Nukiwa, Akira Watanabe

    Research output: Contribution to journalArticlepeer-review

    16 Citations (Scopus)

    Abstract

    An aminoglycoside-modifying enzyme in arbekacin-resistant methicillin- resistant Staphylococcus aureus (MRSA), exhibiting 4'''-N-acetylation, was examined. Although the MRSA strain with AAC(4''') had no AAC(6')-APH(2'') activity, a DNA fragment of the AAC(6')-APH(2'') gene was amplified by PCR and the purified N-terminal 30-amino acid sequence of this AAC(4''') was identical to AAC(6')-APH(2''). Direct DNA sequencing of this 'silent' AAC(6')-APH(2'') gene revealed a single point mutation leading to a substitution of Gly for Asp80, through which the secondary structure is affected. A change in protein conformation could lead to a cleavage and a change of the enzymatic activity. We propose a new aminoglycoside-resistance mediated by AAC(4''') is caused by a mutation-modified AAC(6')-APH(2''). (C) 2000 Federation of European Microbiological Societies.

    Original languageEnglish
    Pages (from-to)299-303
    Number of pages5
    JournalFEMS Microbiology Letters
    Volume190
    Issue number2
    DOIs
    Publication statusPublished - 2000 Sep 15

    Keywords

    • Amikacin
    • Aminoglycoside acetyltransferase
    • Aminoglycoside resistance
    • Arbekacin
    • Methicillin-resistant Staphylococcus aureus

    ASJC Scopus subject areas

    • Microbiology
    • Molecular Biology
    • Genetics

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