Novel actin-related proteins in vertebrates: Similarities of structure and expression pattern to Arp6 localized on Drosophila heterochromatin

Megumi Kato, Mitsuho Sasaki, Shigeki Mizuno, Masahiko Harata

    Research output: Contribution to journalArticlepeer-review

    34 Citations (Scopus)

    Abstract

    Actin-related proteins (Arps), which share a basal structure with actin isoforms but possess different functions, have been identified in a wide variety of organisms. The Arps are classified into subfamilies based on the relatedness of their sequences and functions. Recently, several Arp subfamilies have been shown to be localized in the nucleus and included in protein complexes involved in the organization of chromatin structure, for example, in chromatin remodeling and histone acetyltransferase complexes. A member of the Arp6 subfamily in Drosophila, dArp6, is localized on centric heterochromatin together with heterochromatin protein 1 (HP1). We have identified the first examples of the Arp6 subfamily in vertebrates, novel human and chicken Arps, hArp6 and gArp6, respectively. They are closely related to each other (98% similar) and show apparent similarity to dArp6 (70%). In addition, the hArp6 gene possesses evolutionarily conserved exon/intron structures compared with genes for members of the Arp6 subfamily in invertebrates. Like Drosophila dArp6, gArp6 is expressed abundantly in the early developmental stages, when heterochromatin condensation and nuclear maturation occur. The finding of a conserved Arp6 subfamily in vertebrates will contribute to the understanding of molecular mechanisms of heterochromatin organization.

    Original languageEnglish
    Pages (from-to)133-140
    Number of pages8
    JournalGene
    Volume268
    Issue number1-2
    DOIs
    Publication statusPublished - 2001 May 2

    Keywords

    • Chromatin structure
    • Early embryogenesis
    • Exon/intron structure
    • Gene silencing
    • Heterochromatin protein 1

    ASJC Scopus subject areas

    • Genetics

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