Notch signaling partly regulates the osteogenic differentiation of retinoic acid-treated murine induced pluripotent stem cells

Thanaphum Osathanon, Jeeranan Manokawinchoke, Hiroshi Egusa, Prasit Pavasant

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Notch signaling is involved in osteogenic differentiation; however, its role differs depending on cell type and differentiation stage. Here, we investigated the involvement of Notch signaling in the osteogenic differentiation of retinoic acid-treated embryoid bodies derived from mouse gingival fibroblast-derived induced pluripotent stem cells (mGF-iPSCs). When cultured in osteogenic media, mGF-iPSCs showed an increase in their expression of osteogenic marker genes and deposited a mineralized matrix. Furthermore, increased levels of mRNA for Notch1, Notch2, and Hey1 were observed. In the presence of DAPT, a Notch signaling inhibitor, during osteogenic induction, mRNA levels for osteogenic marker genes were significantly decreased; however, no difference was noted in mineral deposition. Moreover, activation of Notch signaling using Jagged1-immobilized surfaces resulted in a slight increase of in vitro mineralization on days 3 and 7 of osteogenic induction. Significant upregulation of Dlx5, Bsp, and Col I mRNA expression was observed in mGF-iPSCs cultured on Jagged1 surfaces. In conclusion, inhibition and activation of Notch signaling was shown to decrease and increase mGF-iPSC osteogenic differentiation, respectively. However, the responses were not robust, suggesting the involvement of additional signaling pathways.

Original languageEnglish
Pages (from-to)405-413
Number of pages9
JournalJournal of Oral Science
Volume59
Issue number3
DOIs
Publication statusPublished - 2017

Keywords

  • Induced pluripotent stem cells
  • Jagged1
  • Notch signaling
  • Osteogenic differentiation

ASJC Scopus subject areas

  • Dentistry(all)

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