Notch signaling is necessary for GATA3 function in the initiation of T cell development

Katsuto Hozumi, Naoko Negishi, Izumi Tsuchiya, Natsumi Abe, Ken Ichi Hirano, Daisuke Suzuki, Masayuki Yamamoto, James D. Engel, Sonoko Habu

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

GATA3 and Notch1 are essential for T cell development at the earliest stage, but their mutual roles in this process remain to be clarified. In this study, we demonstrated that impairment of T lymphopoiesis in hematopoietic progenitor cells (HPC) of GATA3-deficient fetal liver (FL) on day 11.5 of gestation (E11.5) was rescued only by introduction of both GATA3 and the intracellular region of Notch1 but not by either alone. However, the introduclion of GATA3 only was sufficient for T cell induction in GATA3-deficient FL cells at the advanced stage, where Notch signaling is well detectable. This indicates that Notch signaling is necessary for GATA3 to function for T cell fate specification but is not sufficient without GATA3. On the other hand, Notch signaling is sufficient for blockage of B cell development without GATA3, suggesting that T cell fate specification at the branching point does not result simply from the developmental arrest of B cell lineage by Notch signaling.

Original languageEnglish
Pages (from-to)977-985
Number of pages9
JournalEuropean Journal of Immunology
Volume38
Issue number4
DOIs
Publication statusPublished - 2008 Apr

Keywords

  • GATA3
  • Notch signaling
  • Thymopoiesis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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