Nosocomial spread of armA-mediated high-level aminoglycoside resistance in Enterobacteriaceae isolates producing CTX-M-3 β-lactamase in a cancer hospital in Bulgaria

Stefana Sabtcheva, T. Saga, T. Kantardjiev, M. Ivanova, Y. Ishii, M. Kaku

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

1,310 Enterobacteriaceae, 242 Pseudomonas aeruginosa and 97 Acinetobacter baumannii clinical isolates collected at a cancer hospital in Bulgaria were screened for the presence of 16S rRNA methylases. The armA methylase gene was identified in 20 (1.5%) Enterobacteriaceae (7 Klebsiella pneumoniae, 3 Escherichia coli, 3 Serratia marcescens, 3 Citrobacter freundii, 3 Enterobacter cloacae and 1 Klebsiella oxytoca). ArmA-mediated aminoglycoside resistance was transferable by conjugation and carried by closely related IncL/M plasmids which also carried ant3″9, dfrXII, sul1, blaTEM-1, and blaCTX-M-3 genes encoding resistance to streptomycin-spectinomycin, trimethoprim, sulfonamides, and β-lactams, respectively. Most of the isolates were genetically different according to PFGE but shared similar restriction patterns of the armA-encoding plasmids. Our findings highlight the strong association of armA and blaCTX-M-3 extended-spectrum β-lactamase genes across various species in the family Enterobacteriaceae. The spread of multiresistant isolates expressing 16S rRNA methylases and ESBLs is a worrisome development requiring continuous monitoring.

Original languageEnglish
Pages (from-to)593-599
Number of pages7
JournalJournal of Chemotherapy
Volume20
Issue number5
DOIs
Publication statusPublished - 2008 Oct

Keywords

  • 16S rRNA methylase
  • Aminoglycoside resistance
  • ArmA
  • Enterobacteriaceae

ASJC Scopus subject areas

  • Oncology
  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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