Normal human fibroblasts immortalized by introduction of human papillomavirus type 16 (HPV-16) E6-E7 genes

Takashi Shiga, Hiroshi Shirasawa, Kumiko Shimizu, Mari Dezawa, Yoshiaki Masuda, Bunsiti Simizu

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

This report demonstrates that normal human fibroblasts can be immortalized by the introduction of HPV-16 E6-E7 genes. We designed zinc- inducible expression plasmids with HPV-16 E6, E7 or both. Each plasmid was introduced into normal human fibroblasts (TIG-3 cells) using lipofection methods. Only transfectants with the HPV-16 E6-E7 zinc-inducible expression plasmid, which were cultured in medium supplemented with 100 μM ZnSO4, overcame crisis and could be cultured over 200 population doubling levels (PDLs). These cell lines showed the reactivation of telomerase after crisis, and morphological alterations were also observed.

Original languageEnglish
Pages (from-to)313-319
Number of pages7
JournalMICROBIOLOGY and IMMUNOLOGY
Volume41
Issue number4
DOIs
Publication statusPublished - 1997
Externally publishedYes

Keywords

  • Human fibroblasts
  • Human papillomavirus type 16 (HPV-16)
  • Immortalization

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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