Nonstatistical binding of a protein to clustered carbohydrates

Nina Horan, Lin Yan, Hiroyuki Isobe, George M. Whitesides, Daniel Kahne

    Research output: Contribution to journalArticle

    185 Citations (Scopus)

    Abstract

    Carbohydrate-derivatized self-assembled monolayers (SAMs) are used as a model system to address issues involving cell-surface carbohydrate-protein interactions. Here we examine the influence of carbohydrate surface density on protein-binding avidity. We show that the binding selectivity of Bauhinia purpurea lectin switches from one carbohydrate ligand to another as the surface density of the carbohydrate ligands increases from values of χ(sugar) ≃ 0.1-1.0. Polyvalent binding is possible at all surface densities investigated; hence, the switch in selectivity is not due simply to the achievement of a critical density that permits polyvalent contacts. Instead, secondary interactions at high surface densities promote a switch in carbohydrate-binding selectivity. These findings may have implications for how changes in the composition and the density of cell-surface carbohydrates influence biological recognition processes and regulatory pathways.

    Original languageEnglish
    Pages (from-to)11782-11786
    Number of pages5
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume96
    Issue number21
    DOIs
    Publication statusPublished - 1999 Oct 12

    ASJC Scopus subject areas

    • General

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