Nonoptically probing near-field microscopy for the observation of biological living specimens

Yoshimasa Kawata, Manabu Murakami, Chikara Egami, Okihiro Sugihara, Naomichi Okamoto, Masaaki Tsuchimori, Osamu Watanabe, Osamu Nakamura

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


We present the observation of living specimens with subwavelength resolution by using the nonoptically probing near-field microscopy we have developed recently. In the near-field microscope, the optical field distributions near the specimens are recorded as the surface topography of a photosensitive film, and the topographical distributions are readout with an atomic-force microscopy. Since the near-field microscope does not require the scanning of a probe tip for illumination or detection or scattering of light, it is possible to observe moving biological specimens and fast phenomena. We demonstrate the observation of a moving paramecium and euglena gracilis with subwavelength resolution. The observation of the nucleus inside a euglena cell was also demonstrated.

Original languageEnglish
Pages (from-to)2247-2249
Number of pages3
JournalApplied Physics Letters
Issue number15
Publication statusPublished - 2001 Apr 9

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)


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