Nonequilibrium energetics of a single F1-ATPase molecule

Shoichi Toyabe, Tetsuaki Okamoto, Takahiro Watanabe-Nakayama, Hiroshi Taketani, Seishi Kudo, Eiro Muneyuki

Research output: Contribution to journalArticlepeer-review

112 Citations (Scopus)

Abstract

Molecular motors drive mechanical motions utilizing the free energy liberated from chemical reactions such as ATP hydrolysis. Although it is essential to know the efficiency of this free energy transduction, it has been a challenge due to the system's microscopic scale. Here, we evaluate the single-molecule energetics of a rotary molecular motor, F1-ATPase, by applying a recently derived nonequilibrium equality together with an electrorotation method. We show that the sum of the heat flow through the probe's rotational degree of freedom and the work against an external load is almost equal to the free energy change per a single ATP hydrolysis under various conditions. This implies that F1-ATPase works at an efficiency of nearly 100% in a thermally fluctuating environment.

Original languageEnglish
Article number198103
JournalPhysical Review Letters
Volume104
Issue number19
DOIs
Publication statusPublished - 2010 May 14

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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