TY - JOUR
T1 - Noncompetitive Fluorescence Polarization Immunoassay for Protein Determination
AU - Fukuyama, Mao
AU - Nakamura, Ayano
AU - Nishiyama, Keine
AU - Imai, Ayuko
AU - Tokeshi, Manabu
AU - Shigemura, Koji
AU - Hibara, Akihide
N1 - Funding Information:
This study was supported by the JST-SENTAN program JPMJSN16A2 and by the Research Program of the “Dynamic Alliance for Open Innovation Bridging Human, Environment and Materials” in the “Network Joint Research Centre for Materials and Devices”. M.F. sincerely appreciates Prof. S. Mizukami, Prof. T. Matsui, and K. Sakai for the technical support.
PY - 2020/11/3
Y1 - 2020/11/3
N2 - Fluorescent polarization immunoassay (FPIA) is a single-step immunoassay method that is applicable to point-of-care testing; however, its applicability to large biomolecules has been restricted because ordinary FPIA is a competitive assay. Here, we report a noncompetitive FPIA using the variable domain from the heavy chain of a camelid antibody (VHH antibody). FPIA with VHH was successfully used to quantitate rabbit immunoglobulin G (IgG) and demonstrated a wider response range than that observed with antibody-binding (Fab) fragment. Then, using a portable FPIA instrument, a VHH-based immunoassay of human IgG in a human serum certified reference material was demonstrated.
AB - Fluorescent polarization immunoassay (FPIA) is a single-step immunoassay method that is applicable to point-of-care testing; however, its applicability to large biomolecules has been restricted because ordinary FPIA is a competitive assay. Here, we report a noncompetitive FPIA using the variable domain from the heavy chain of a camelid antibody (VHH antibody). FPIA with VHH was successfully used to quantitate rabbit immunoglobulin G (IgG) and demonstrated a wider response range than that observed with antibody-binding (Fab) fragment. Then, using a portable FPIA instrument, a VHH-based immunoassay of human IgG in a human serum certified reference material was demonstrated.
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U2 - 10.1021/acs.analchem.0c02300
DO - 10.1021/acs.analchem.0c02300
M3 - Article
AN - SCOPUS:85095817280
VL - 92
SP - 14393
EP - 14397
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 21
ER -