TY - JOUR
T1 - Noncompetitive Fluorescence Polarization Immunoassay for Protein Determination
AU - Fukuyama, Mao
AU - Nakamura, Ayano
AU - Nishiyama, Keine
AU - Imai, Ayuko
AU - Tokeshi, Manabu
AU - Shigemura, Koji
AU - Hibara, Akihide
N1 - Publisher Copyright:
©
PY - 2020/11/3
Y1 - 2020/11/3
N2 - Fluorescent polarization immunoassay (FPIA) is a single-step immunoassay method that is applicable to point-of-care testing; however, its applicability to large biomolecules has been restricted because ordinary FPIA is a competitive assay. Here, we report a noncompetitive FPIA using the variable domain from the heavy chain of a camelid antibody (VHH antibody). FPIA with VHH was successfully used to quantitate rabbit immunoglobulin G (IgG) and demonstrated a wider response range than that observed with antibody-binding (Fab) fragment. Then, using a portable FPIA instrument, a VHH-based immunoassay of human IgG in a human serum certified reference material was demonstrated.
AB - Fluorescent polarization immunoassay (FPIA) is a single-step immunoassay method that is applicable to point-of-care testing; however, its applicability to large biomolecules has been restricted because ordinary FPIA is a competitive assay. Here, we report a noncompetitive FPIA using the variable domain from the heavy chain of a camelid antibody (VHH antibody). FPIA with VHH was successfully used to quantitate rabbit immunoglobulin G (IgG) and demonstrated a wider response range than that observed with antibody-binding (Fab) fragment. Then, using a portable FPIA instrument, a VHH-based immunoassay of human IgG in a human serum certified reference material was demonstrated.
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U2 - 10.1021/acs.analchem.0c02300
DO - 10.1021/acs.analchem.0c02300
M3 - Article
AN - SCOPUS:85095817280
VL - 92
SP - 14393
EP - 14397
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 21
ER -