Non-reductive scavenging of 1,1-diphenyl-2-picrylhydrazyl (DPPH) by peroxyradical: A useful method for quantitative analysis of peroxyradical

Masahiro Nishizawa, Masahiro Kohno, Minemitsu Nishimura, Akio Kitagawa, Yoshimi Niwano

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

A stable radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) has long been used as a convenient method for the antioxidant assay of biological materials such as cysteine, glutathione, ascorbic acid, tocopherol and polyhydroxy aromatic compounds (hydroquinone, pyrogallol, etc). In this study, non-reductive scavenging of DPPH was investigated by electron spin resonance (ESR) analyses for the purpose of developing a useful method for quantitative determination of peroxyradical. Since DPPH was degraded in the presence of peroxyradical derived from UV-irradiated benzoylperoxide and the peroxyradical-induced degradation of DPPH was inhibited by the addition of a spin trapping agent 5,5-dimethyl-1- pyrroline-N-oxide (DMPO), it is concluded that DPPH is non-reductively scavenged by peroxyradical. Therefore, it is suggested that DPPH could be a useful agent for the quantitative measurement of peroxyradical.

Original languageEnglish
Pages (from-to)714-716
Number of pages3
JournalChemical and Pharmaceutical Bulletin
Volume53
Issue number6
DOIs
Publication statusPublished - 2005 Jun

Keywords

  • 1,1-dipheny 1-2-picryihydrazyl (DPPH)
  • Electron spin resonance (ESR)
  • Non-reductive scavenging
  • Peroxyradical

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

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