TY - JOUR
T1 - Non-reductive scavenging of 1,1-diphenyl-2-picrylhydrazyl (DPPH) by peroxyradical
T2 - A useful method for quantitative analysis of peroxyradical
AU - Nishizawa, Masahiro
AU - Kohno, Masahiro
AU - Nishimura, Minemitsu
AU - Kitagawa, Akio
AU - Niwano, Yoshimi
PY - 2005/6
Y1 - 2005/6
N2 - A stable radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) has long been used as a convenient method for the antioxidant assay of biological materials such as cysteine, glutathione, ascorbic acid, tocopherol and polyhydroxy aromatic compounds (hydroquinone, pyrogallol, etc). In this study, non-reductive scavenging of DPPH was investigated by electron spin resonance (ESR) analyses for the purpose of developing a useful method for quantitative determination of peroxyradical. Since DPPH was degraded in the presence of peroxyradical derived from UV-irradiated benzoylperoxide and the peroxyradical-induced degradation of DPPH was inhibited by the addition of a spin trapping agent 5,5-dimethyl-1- pyrroline-N-oxide (DMPO), it is concluded that DPPH is non-reductively scavenged by peroxyradical. Therefore, it is suggested that DPPH could be a useful agent for the quantitative measurement of peroxyradical.
AB - A stable radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) has long been used as a convenient method for the antioxidant assay of biological materials such as cysteine, glutathione, ascorbic acid, tocopherol and polyhydroxy aromatic compounds (hydroquinone, pyrogallol, etc). In this study, non-reductive scavenging of DPPH was investigated by electron spin resonance (ESR) analyses for the purpose of developing a useful method for quantitative determination of peroxyradical. Since DPPH was degraded in the presence of peroxyradical derived from UV-irradiated benzoylperoxide and the peroxyradical-induced degradation of DPPH was inhibited by the addition of a spin trapping agent 5,5-dimethyl-1- pyrroline-N-oxide (DMPO), it is concluded that DPPH is non-reductively scavenged by peroxyradical. Therefore, it is suggested that DPPH could be a useful agent for the quantitative measurement of peroxyradical.
KW - 1,1-dipheny 1-2-picryihydrazyl (DPPH)
KW - Electron spin resonance (ESR)
KW - Non-reductive scavenging
KW - Peroxyradical
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U2 - 10.1248/cpb.53.714
DO - 10.1248/cpb.53.714
M3 - Article
C2 - 15930791
AN - SCOPUS:21144456671
VL - 53
SP - 714
EP - 716
JO - Chemical and Pharmaceutical Bulletin
JF - Chemical and Pharmaceutical Bulletin
SN - 0009-2363
IS - 6
ER -