Abstract
Pediococcus halophilus possesses phosphoenolpyruvate:mannose phosphotransferase system (man:PTS) as a main glucose transporter. A man:PTS defective (man:PTSd) strain X-160 could, however, utilize glucose. A possible glucose-transport mechanism other than PTS was studied with the strain X-160 and its derivative, man:PTSd phosphofructokinase defective (PFK-) strain M-13. Glucose uptake by X-160 at pH 5.5 was inhibited by any of carbonylcyanide m-chlorophenylhydrazone, nigericin, N,N′-dicyclohexylcarbodiimide, or iodoacetic acid. The double mutant M-13 could still transport glucose and accumulated intracellularly a large amount of hexose-phosphates (ca. 8 mM glucose 6-phosphate and ca. 2 mM fructose 6-phosphate). Protonophores also inhibited the glucose transport at pH 5.5, as determined by the amounts of accumulated hexose-phosphates (< 4 mM). These showed involvement of proton motive force (ΔP) in the non-PTS glucose transport. It was concluded that the non-PTS glucose transporter operated in concert with hexokinase or glucokinase for the metabolism of glucose in the man:PTSd strain.
Original language | English |
---|---|
Pages (from-to) | 537-540 |
Number of pages | 4 |
Journal | Archives of Microbiology |
Volume | 153 |
Issue number | 6 |
DOIs | |
Publication status | Published - 1990 May 1 |
Externally published | Yes |
Keywords
- Glucokinase
- Glucose transport
- Hexokinase
- Pediococcus halophilus
- Phosphofructokinase
- Phsophotransferase
- Proton motive force
ASJC Scopus subject areas
- Microbiology
- Biochemistry
- Molecular Biology
- Genetics