Non-cell-autonomous control of vascular stem cell fate by a CLE peptide/receptor system

Yuki Hirakawa, Hidefumi Shinohara, Yuki Kondo, Asuka Inoue, Ikuko Nakanomyo, Mari Ogawa, Shinichiro Sawa, Kyoko Ohashi-Ito, Yoshikatsu Matsubayashi, Hiroo Fukuda

Research output: Contribution to journalArticlepeer-review

321 Citations (Scopus)

Abstract

Land plants evolved a long-distance transport system of water and nutrients composed of the xylem and phloem, both of which are generated from the procambium- and cambium-comprising vascular stem cells. However, little is known about the molecular mechanism of cell communication governing xylem-phloem patterning. Here, we show that a dodecapeptide (HEVHypSGHypNPISN; Hyp, 4-hydroxyproline), TDIF (tracheary element differentiation inhibitory factor), is secreted from the phloem and suppresses the differentiation of vascular stem cells into xylem cells through a leucine-rich repeat receptor-like kinase (LRR-RLK). TDIF binds in vitro specifically to the LRR-RLK, designated TDR (putative TDIF receptor), whose expression is restricted to procambial cells. However, the combined analysis of TDIF with a specific antibody and the expression profiles of the promoters of two genes encoding TDIF revealed that TDIF is synthesized mainly in, and secreted from, the phloem and its neighboring cells. The observation that TDIF is capable of promoting proliferation of procambial cells while suppressing xylem differentiation suggests that this small peptide functions as a phloem-derived, non-cellautonomous signal that controls stem cell fate in the procambium. Our results indicate that we have discovered a cell communication system governing phloem-xylem cross-talk.

Original languageEnglish
Pages (from-to)15208-15213
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number39
DOIs
Publication statusPublished - 2008 Sep 30
Externally publishedYes

Keywords

  • CLV3/ESR-related (CLE)
  • Leucine-rich repeat receptor-like kinase
  • Phloem
  • Procambium
  • Xylem

ASJC Scopus subject areas

  • General

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