Nocodazole induces mitotic cell death with apoptotic-like features in Saccharomyces cerevisiae

Kingo Endo, Mayumi Mizuguchi, Aoi Harata, Go Itoh, Kozo Tanaka

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

We investigated the fate of budding yeast treated with nocodazole, a microtubule-depolymerizing drug. Cells died after mitotic arrest while staying in mitosis, suggesting that mitotic cell death, but not mitotic slippage, mainly occurs in nocodazole-treated cells. Nocodazole-treated cells showed features of apoptotic-like cell death, but not those of cell lysis or autophagy. Consistently, mitochondria-dependent production of reactive oxygen species was involved in the cell death. Similar cell death was also seen in cells after mitotic arrest by perturbation of the anaphase-promoting complex/cyclosome. In addition, caspase activity was found in nocodazole-treated cells, which was independent of the metacaspase, Mca1. Our results suggest that budding yeast can be a model to study mitotic cell death in cancer treatment with antimitotic drugs.

Original languageEnglish
Pages (from-to)2387-2392
Number of pages6
JournalFEBS Letters
Volume584
Issue number11
DOIs
Publication statusPublished - 2010 Jun

Keywords

  • Apoptosis
  • Budding yeast
  • Mitotic arrest
  • Mitotic cell death
  • Nocodazole

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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