N,N,N-trimethylsphingosine inhibits interleukin-1β-induced NF-κB activation and consequent E-selectin expression in human umbilical vein endothelial cells

Atsushi Masamune; Sen itiroh Hakomori; Yasuyuki Igarashi

doi:10.1016/0014-5793(95)00566-R

N,N,N-trimethylsphingosine inhibits interleukin-1β-induced NF-κB activation and consequent E-selectin expression in human umbilical vein endothelial cells

Atsushi Masamune, Sen itiroh Hakomori, Yasuyuki Igarashi

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

We examined the effect of N,N,N-trimethylsphingosine (TMS) on the interleukin-1β (IL-1β)-induced E-selectin expression in human umbilical vein endothelial cells (HUVEC). Incubation of HUVEC with TMS (0.1-10 μM) resulted in a concentration-dependent inhibition of IL-1β-induced E-selectin expression. Sphingosine or N,N-dimethylsphingosine had no effects on the expression. Electrophoretic mobility shift assay revealed that TMS inhibited IL-1β-induced NF-κB activation, which is essential for E-selectin expression. This inhibitory effect of TMS on IL-1β-dependent endothelial cell activation may partly explain the known anti-inflammatory or anti-metastatic effect of TMS in vivo.

Original language	English
Pages (from-to)	205-209
Number of pages	5
Journal	FEBS Letters
Volume	367
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(95)00566-R
Publication status	Published - 1995 Jun 26
Externally published	Yes

Keywords

E-selectin
Interleukin-1β
NF-κB
Sphingosine

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "N,N,N-trimethylsphingosine inhibits interleukin-1β-induced NF-κB activation and consequent E-selectin expression in human umbilical vein endothelial cells",

abstract = "We examined the effect of N,N,N-trimethylsphingosine (TMS) on the interleukin-1β (IL-1β)-induced E-selectin expression in human umbilical vein endothelial cells (HUVEC). Incubation of HUVEC with TMS (0.1-10 μM) resulted in a concentration-dependent inhibition of IL-1β-induced E-selectin expression. Sphingosine or N,N-dimethylsphingosine had no effects on the expression. Electrophoretic mobility shift assay revealed that TMS inhibited IL-1β-induced NF-κB activation, which is essential for E-selectin expression. This inhibitory effect of TMS on IL-1β-dependent endothelial cell activation may partly explain the known anti-inflammatory or anti-metastatic effect of TMS in vivo.",

keywords = "E-selectin, Interleukin-1β, NF-κB, Sphingosine",

author = "Atsushi Masamune and Hakomori, {Sen itiroh} and Yasuyuki Igarashi",

year = "1995",

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doi = "10.1016/0014-5793(95)00566-R",

language = "English",

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T1 - N,N,N-trimethylsphingosine inhibits interleukin-1β-induced NF-κB activation and consequent E-selectin expression in human umbilical vein endothelial cells

AU - Masamune, Atsushi

AU - Hakomori, Sen itiroh

AU - Igarashi, Yasuyuki

PY - 1995/6/26

Y1 - 1995/6/26

N2 - We examined the effect of N,N,N-trimethylsphingosine (TMS) on the interleukin-1β (IL-1β)-induced E-selectin expression in human umbilical vein endothelial cells (HUVEC). Incubation of HUVEC with TMS (0.1-10 μM) resulted in a concentration-dependent inhibition of IL-1β-induced E-selectin expression. Sphingosine or N,N-dimethylsphingosine had no effects on the expression. Electrophoretic mobility shift assay revealed that TMS inhibited IL-1β-induced NF-κB activation, which is essential for E-selectin expression. This inhibitory effect of TMS on IL-1β-dependent endothelial cell activation may partly explain the known anti-inflammatory or anti-metastatic effect of TMS in vivo.

AB - We examined the effect of N,N,N-trimethylsphingosine (TMS) on the interleukin-1β (IL-1β)-induced E-selectin expression in human umbilical vein endothelial cells (HUVEC). Incubation of HUVEC with TMS (0.1-10 μM) resulted in a concentration-dependent inhibition of IL-1β-induced E-selectin expression. Sphingosine or N,N-dimethylsphingosine had no effects on the expression. Electrophoretic mobility shift assay revealed that TMS inhibited IL-1β-induced NF-κB activation, which is essential for E-selectin expression. This inhibitory effect of TMS on IL-1β-dependent endothelial cell activation may partly explain the known anti-inflammatory or anti-metastatic effect of TMS in vivo.

KW - E-selectin

KW - Interleukin-1β

KW - NF-κB

KW - Sphingosine

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JF - FEBS Letters

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ER -

N,N,N-trimethylsphingosine inhibits interleukin-1β-induced NF-κB activation and consequent E-selectin expression in human umbilical vein endothelial cells

Abstract

Keywords

ASJC Scopus subject areas

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