N,N,N-trimethylsphingosine inhibits interleukin-1β-induced NF-κB activation and consequent E-selectin expression in human umbilical vein endothelial cells

Atsushi Masamune, Sen itiroh Hakomori, Yasuyuki Igarashi

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

We examined the effect of N,N,N-trimethylsphingosine (TMS) on the interleukin-1β (IL-1β)-induced E-selectin expression in human umbilical vein endothelial cells (HUVEC). Incubation of HUVEC with TMS (0.1-10 μM) resulted in a concentration-dependent inhibition of IL-1β-induced E-selectin expression. Sphingosine or N,N-dimethylsphingosine had no effects on the expression. Electrophoretic mobility shift assay revealed that TMS inhibited IL-1β-induced NF-κB activation, which is essential for E-selectin expression. This inhibitory effect of TMS on IL-1β-dependent endothelial cell activation may partly explain the known anti-inflammatory or anti-metastatic effect of TMS in vivo.

Original languageEnglish
Pages (from-to)205-209
Number of pages5
JournalFEBS Letters
Volume367
Issue number2
DOIs
Publication statusPublished - 1995 Jun 26
Externally publishedYes

Keywords

  • E-selectin
  • Interleukin-1β
  • NF-κB
  • Sphingosine

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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