NMR spectroscopic study of a DNA duplex with mercury-mediated T-T base pairs

Yoshiyuki Tanaka, Hiroshi Yamaguchi, Shuji Oda, Yoshinori Kondo, Makoto Nomura, Chojiro Kojima, Akira Ono

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Recently, we reported that T-T mismatches can specifically recognize HgII (T-HgII-T pair formation). In order to understand the properties of the T-HgII-T pair, we recorded NMR spectra for a DNA duplex, d(CGCGTTGTCC) • d(GGACTTCGCG), with two successive T-T mismatches (HgII-binding sites). We assigned 1H resonances for mercury-free and di-mercurated duplexes, and performed titration experiments with HgII by using 1D 1H NMR spectra. Because of the above mentioned assignments, we could confirm the existence of mono-mercurated species, because individual components gave independent NMR signals in the titration spectra.

Original languageEnglish
Pages (from-to)613-624
Number of pages12
JournalNucleosides, Nucleotides and Nucleic Acids
Volume25
Issue number4-6
DOIs
Publication statusPublished - 2006 Jun 1

Keywords

  • Equilibrium
  • H NMR
  • Hg-mediated T-T base pair
  • Titration

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Genetics

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