Nitrite Production from Nitrate and Its Link with Lactate Metabolism in Oral Veillonella spp.

Dimas Prasetianto Wicaksono, Jumpei Washio, Yuki Abiko, Hitomi Domon, Nobuhiro Takahashia

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Veillonella species are among the major anaerobes in the oral cavity and are frequently detected in both caries lesions and healthy oral microbiomes. They possess the ability to utilize lactate and convert nitrate (NO3-) into nitrite (NO2- ). Recently, interest in NO2- has increased rapidly because of its beneficial ef-fects on oral and general health; i.e., it inhibits the growth and metabolism of oral pathogenic bacteria, such as Streptococcus mutans, and lowers systemic blood pres-sure. However, there is only limited information about the biochemical characteris-tics of NO2- production by Veillonella species. We found that NO3- did not inhibit the growth of Veillonella atypica or Veillonella parvula, and it inhibited the growth of Streptococcus mutans only at a high concentration (100 mM). However, NO2- inhib-ited the growth of Streptococcus mutans at a low concentration (0.5 mM), while a higher concentration of NO2- (20 mM) was needed to inhibit the growth of Veillo-nella species. NO2- production by Veillonella species was increased by environmental factors (lactate, acidic pH, and anaerobic conditions) and growth conditions (the presence of NO3- or NO2-) and was linked to anaerobic lactate metabolism. A stoi-chiometric evaluation revealed that NO3- is reduced to NO2- by accepting reducing power derived from the oxidization of lactate. These findings suggest that the bio-chemical characteristics of NO2- production from NO3- and its linkage with lactate metabolism in oral Veillonella species may play a key role in maintaining good oral and general health.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalApplied and environmental microbiology
Volume86
Issue number20
DOIs
Publication statusPublished - 2020 Oct

Keywords

  • Veillonella
  • diet
  • lactate
  • metabolism
  • nitrate
  • nitrite

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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