Nitrite-producing oral microbiome in adults and children

Yuria Sato-Suzuki, Jumpei Washio, Dimas Prasetianto Wicaksono, Takuichi Sato, Satoshi Fukumoto, Nobuhiro Takahashi

Research output: Contribution to journalArticlepeer-review

Abstract

Recently, it was suggested that the nitrite (NO2) produced from NO3 by oral bacteria might contribute to oral and general health. Therefore, we aimed to clarify the detailed information about the bacterial NO2-production in the oral biofilm. Dental plaque and tongue-coating samples were collected, then the NO2-producing activity was measured. Furthermore, the composition of the NO2-producing bacterial population were identified using the Griess reagent-containing agar overlay method and molecular biological method. NO2-producing activity per mg wet weight varied among individuals but was higher in dental plaque. Additionally, anaerobic bacteria exhibited higher numbers of NO2-producing bacteria, except in the adults’ dental plaque. The proportion of NO2-producing bacteria also varied among individuals, but a positive correlation was found between NO2-producing activity and the number of NO2-producing bacteria, especially in dental plaque. Overall, the major NO2-producing bacteria were identified as Actinomyces, Schaalia, Veillonella and Neisseria. Furthermore, Rothia was specifically detected in the tongue coatings of children. These results suggest that dental plaque has higher NO2-producing activity and that this activity depends not on the presence of specific bacteria or the bacterial compositions, but on the number of NO2-producing bacteria, although interindividual differences were detected.

Original languageEnglish
Article number16652
JournalScientific reports
Volume10
Issue number1
DOIs
Publication statusPublished - 2020 Dec 1

ASJC Scopus subject areas

  • General

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