Nitric oxide synthases and heart failure - lessons from genetically manipulated mice

Kiyoko Shibata, Hiroaki Shimokawa, Nobuyuki Yanagihara, Yutaka Otsuji, Masato Tsutsui

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


Nitric oxide (NO) is synthesized by three distinct NO synthase (NOS) isoforms (neuronal, inducible, and endothelial NOS), all of which are expressed in the human heart. The roles of NOSs in the pathogenesis of heart failure have been described in pharmacological studies with NOS inhibitors. Recently, genetically engineered animals have been used. We have generated mice in which all 3 NOS isoforms are completely disrupted (triple n/i/eNOS-/- mice). Morphological, echocardiographic, and hemodynamic analysis were performed in wild-type, singly nNOS-/-, iNOS-/-, eNOS-/-, and triple n/i/eNOS-/- mice. Importantly, significant left ventricular (LV) hypertrophy and diastolic dysfunction was noted only in n/i/eNOS-/- mice, and those pathology was similar to diastolic heart failure in humans. Finally, treatment with an angiotensin II type 1 (AT1) receptor blocker, significantly prevented those abnormalities. These results provide the evidence that AT1 receptor pathway plays a center role in the pathogenesis of cardiac disorders in the n/i/eNOS-/- mice. Our studies with triple n/i/eNOS-/- mice provide pivotal insights into an understanding of the pathophysiology of NOSs in human heart failure.

Original languageEnglish
Pages (from-to)147-158
Number of pages12
JournalJournal of UOEH
Issue number2
Publication statusPublished - 2013 Jun


  • Heart failure
  • Left ventricular hypertrophy
  • Mice
  • Nitric oxide synthase

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health


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