Nitric oxide synthase (NOS) in the Japanese fireflies Luciola lateralis and Luciola cruciata

Hajime Ohtsuki, Jun Yokoyama, Nobuyoshi Ohba, Yoshihiro Ohmiya, Masakado Kawata

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Species-specific flash patterns in firefly species are important for the investigation of the evolution of Lampyridae. Since nitric oxide synthase (NOS) is one of the key enzymes controlling flash patterns, we determined the cDNA sequences of NOS in the Japanese fireflies Luciola lateralis and L. cruciata. The identity of the NOS sequences was very high between these 2 species. Firefly NOS also exhibited a high identity with those of other insect species, and the cofactor-binding domains were particularly well conserved. Many negatively selected sites were detected throughout the NOS sequences; however, no positive selection was detected. The phylogenetic relationship of insect NOS was different from that of the general classification system, although the lineages corresponded to the major recognized taxonomic groups.

Original languageEnglish
Pages (from-to)176-188
Number of pages13
JournalArchives of Insect Biochemistry and Physiology
Volume69
Issue number4
DOIs
Publication statusPublished - 2008 Dec 1

Keywords

  • Luciola cruciata
  • Luciola lateralis
  • Molecular evolution
  • NO synthase
  • cDNA cloning

ASJC Scopus subject areas

  • Physiology
  • Biochemistry
  • Insect Science

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